Anti-Cytokeratin 14抗体[LL002] - BSA and Azide free (ab212547)
Key features and details
- Mouse monoclonal [LL002] to Cytokeratin 14 - BSA and Azide free
- Suitable for: ICC/IF, WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG3
Related conjugates and formulations
概述
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产品名称
Anti-Cytokeratin 14抗体[LL002] - BSA and Azide free
参阅全部 Cytokeratin 14 一抗 -
描述
小鼠单克隆抗体[LL002] to Cytokeratin 14 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Cytokeratin 14 (C terminal). (15 amino acid peptide).
Database link: P02533 -
阳性对照
- IHC-P: Human prostate tissue. ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
ab212547 was purified from Bioreactor Concentrate by Protein A/G. -
克隆
单克隆 -
克隆编号
LL002 -
同种型
IgG3 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab212547于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 0.1 - 1 µg/ml.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
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IHC-P | (1) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 min at RT). |
说明 |
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ICC/IF
Use a concentration of 0.1 - 1 µg/ml. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 min at RT). |
靶标
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功能
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. -
组织特异性
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. -
疾病相关
Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy. -
序列相似性
Belongs to the intermediate filament family. -
细胞定位
Cytoplasm. Nucleus. Expressed in both as a filamentous pattern. - Information by UniProt
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数据库链接
- Entrez Gene: 3861 Human
- Entrez Gene: 16664 Mouse
- Entrez Gene: 287701 Rat
- Omim: 148066 Human
- SwissProt: P02533 Human
- SwissProt: Q61781 Mouse
- SwissProt: Q6IFV1 Rat
- Unigene: 654380 Human
see all -
别名
- CK 14 antibody
- CK-14 antibody
- ck14 antibody
see all
图片
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Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 14 using ab212547 at 1 µg/ml in immunohistochemical analysis.
This IHC-P data was generated using the same anti-Cytokeratin 14 antibody clone, LL002, in a different buffer formulation (cat# ab7800).
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This data was developed using the same antibody clone in a different buffer formulation (ab7800). ab7800 staining KRT14 in wild-type A431 cells (top panel) and KRT14 knockout A431 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7800 at 1μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 14 using ab212547 at 1 µg/ml in immunohistochemical analysis.
This IHC-P data was generated using the same anti-Cytokeratin 14 antibody clone, LL002, in a different buffer formulation (cat# ab7800).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab212547 被引用在 1 文献中.
- Burlingame EA et al. Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platforms. Cell Rep Methods 1:N/A (2021). PubMed: 34485971