Anti-Cytokeratin 13抗体[AE8] (ab16112)
Key features and details
- Mouse monoclonal [AE8] to Cytokeratin 13
- Suitable for: IHC-Fr, ICC/IF, WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cytokeratin 13抗体[AE8]
参阅全部 Cytokeratin 13 一抗 -
描述
小鼠单克隆抗体[AE8] to Cytokeratin 13 -
宿主
Mouse -
经测试应用
适用于: IHC-Fr, ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A431 whole cell lysate. ICC/IF: A431 cells. IHC-P: Human tonsil. IHC-Fr: Human tonsil.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
This antibody is specific for Cytokeratin 13, which is a marker for oesophageal type differentiation which is expressed by various internal stratified epithelia. -
克隆
单克隆 -
克隆编号
AE8 -
骨髓瘤
P3-X63 Ag8.3 -
同种型
IgG -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16112于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use a concentration of 1 µg/ml.
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ICC/IF | (1) |
Use a concentration of 1 - 5 µg/ml.
PubMed: 25076852 |
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
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IHC-P | (3) |
Use a concentration of 0.05 µg/ml.
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说明 |
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IHC-Fr
Use a concentration of 1 µg/ml. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. PubMed: 25076852 |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. |
IHC-P
Use a concentration of 0.05 µg/ml. |
靶标
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组织特异性
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. -
疾病相关
Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
O-glycosylated; glycans consist of single N-acetylglucosamine residues. - Information by UniProt
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数据库链接
- Entrez Gene: 3860 Human
- Entrez Gene: 16663 Mouse
- Omim: 148065 Human
- SwissProt: P13646 Human
- SwissProt: P08730 Mouse
- Unigene: 654550 Human
- Unigene: 4646 Mouse
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别名
- 47 kDa cytokeratin antibody
- CK-13 antibody
- CK13 antibody
see all
图片
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All lanes : Anti-Cytokeratin 13 antibody [AE8] (ab16112) at 1 µg/ml
Lane 1 : Wild-type A431 cell lysate
Lane 2 : KRT13 knockout A431 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Cytokeratin 13 antibody [AE8] staining at 1 µg/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab16112 was shown to bind specifically to Cytokeratin 13. A band was observed at 51 kDa in wild-type A431 cell lysates with no signal observed at this size in Krt13 knockout cell line ab269483 (knockout cell lysate ab269647). To generate this image, wild-type and Krt13 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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IHC image of Cytokeratin 13 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16112, 0.05 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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IHC image of Cytokeratin 13 staining in a section of frozen normal human tonsil*. The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab16112 at 1µg/ml. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), 1/1000)) (shown in green) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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Anti-Cytokeratin 13 antibody [AE8] (ab16112) at 1 µg/ml + A431 whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 50 kDaThis blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab16112 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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ab16112 staining Cytokeratin 13 in A431 cells. The cells were fixed with Methanol (5min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab16112 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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ab16112 staining Cytokeratin 13 in Human pharynx tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in Tris pH9. Samples were incubated with primary antibody (undiluted) for 1 hour at 20°C. An undiluted HRP-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab16112 被引用在 23 文献中.
- Yao Y et al. Hydroxychloroquine treatment on SARS-CoV-2 receptor ACE2, TMPRSS2 and NRP1 expression in human primary pterygium and conjunctival cells. Exp Eye Res 214:108864 (2022). PubMed: 34826419
- Jung KL et al. Single-cell analysis of Kaposi's sarcoma-associated herpesvirus infection in three-dimensional air-liquid interface culture model. PLoS Pathog 18:e1010775 (2022). PubMed: 35976902
- Uetsuki R et al. The squamous cell carcinoma cell line OM-1 retains both p75-dependent stratified epithelial progenitor potential and cancer stem cell properties. Biochem Biophys Rep 26:101003 (2021). PubMed: 34041369
- Watanabe S et al. Human iPS cells engender corneal epithelial stem cells with holoclone-forming capabilities. iScience 24:102688 (2021). PubMed: 34195566
- Konishi T et al. LRRC8A Expression Influences Growth of Esophageal Squamous Cell Carcinoma. Am J Pathol 189:1973-1985 (2019). PubMed: 31323188