Anti-Cytokeratin 10 抗体 [EP1607IHCY] - Cytoskeleton Marker

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Immunocytochemistry/Immunofluorescence analysis of HACAT cells labelling Cytokeratin 10 with purified ab76318 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling Cytokeratin 10 with ab76318 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32 mins. ab76318 Anti-Cytokeratin 10 antibody [EP1607IHCY] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Cytokeratin 10 with purified ab76318 at 1/5000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal skin tissue labelling Cytokeratin 10 with unpurified ab76318.

Green - CK10, red - PI.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Immunohistochemical analysis of formalin fixed paraffin embedded human skin labelling Cytokeratin 10 with ab76318 at a concentration of 0.08µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32 mins. ab76318 Anti-Cytokeratin 10 antibody [EP1607IHCY] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling Cytokeratin 10 with purified ab76318 at 1/5000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• IHC-P

AbReview32716****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/10000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/250).

This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

• WB

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Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/50000 dilution

All lanes:

Human fetal skin tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 59 kDa

Observed band size: 60 kDa

false

• WB

Unknown

Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/10000 dilution

Lane 1:

HACAT cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 59 kDa

Observed band size: 60 kDa

false

• WB

Unknown

Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/200000 dilution

All lanes:

Mouse skin tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 59 kDa

Observed band size: 60 kDa

false

• WB

Unknown

Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/50000 dilution

All lanes:

Rat skin tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 59 kDa

Observed band size: 60 kDa

false

• WB

Unknown

Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

All lanes:

Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/100000 dilution

All lanes:

HaCat cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 59 kDa

Observed band size: 60 kDa

false