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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly

Anti-Cytohesin 2抗体[10A12] (ab2728)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)

Key features and details

  • Mouse monoclonal [10A12] to Cytohesin 2
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Cytohesin 2抗体[10A12]
    参阅全部 Cytohesin 2 一抗
  • 描述

    小鼠单克隆抗体[10A12] to Cytohesin 2
  • 宿主

    Mouse
  • 特异性

    Detects recombinant human cytohesin-2 (ARNO).
  • 经测试应用

    适用于: ICC/IF, IHC-Pmore details
    不适用于: WB
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (His-tag) corresponding to Human Cytohesin 2.
    Database link: Q99418

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯化说明

    PEG purified IgG1
  • 克隆

    单克隆
  • 克隆编号

    10A12
  • 同种型

    IgG1
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Cytohesin 2 protein (ab134606)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2728于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/20.
IHC-P
1/25 - 1/200.
说明
ICC/IF
1/20.
IHC-P
1/25 - 1/200.
应用说明
Is unsuitable for WB.

靶标

  • 功能

    Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.
  • 组织特异性

    Ubiquitous.
  • 序列相似性

    Contains 1 PH domain.
    Contains 1 SEC7 domain.
  • 结构域

    The PH domain is necessary and sufficient for plasma membrane relocalization.
  • 细胞定位

    Cell membrane. Cytoplasm. Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. Requires also interaction with phosphoinositides for targeting to plasma membrane.
  • Target information above from: UniProt accession Q99418 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 9266 Human
    • Entrez Gene: 19158 Mouse
    • Omim: 602488 Human
    • SwissProt: Q99418 Human
    • SwissProt: P63034 Mouse
    • Unigene: 144011 Human
    • Unigene: 272130 Mouse
    • 别名

      • ARF exchange factor antibody
      • ARF nucleotide binding site opener antibody
      • ARF nucleotide-binding site opener antibody
      • Arno antibody
      • ARNO protein antibody
      • CLM2 antibody
      • CTS18 antibody
      • CTS18.1 antibody
      • CYH2_HUMAN antibody
      • Cyth2 antibody
      • Cytohesin 2 antibody
      • Cytohesin-2 antibody
      • MGC137537 antibody
      • MGC80440 antibody
      • PH antibody
      • PH, SEC7 and coiled-coil domain-containing protein 2 antibody
      • Pleckstrin homology Sec7 and coiled coil domains 2 antibody
      • Pleckstrin homology Sec7 and coiled coil domains protein 2 antibody
      • Protein ARNO antibody
      • PSCD2 antibody
      • PSCD2L antibody
      • PSCD2L, formerly antibody
      • SEC7 and coiled-coil domain-containing protein 2 antibody
      • Sec7 antibody
      • SEC7 homolog B antibody
      • Sec7B antibody
      • SEC7L antibody
      • Sec7p L antibody
      • Sec7p-like antibody
      • Sec7pL antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)

      Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)

      Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)

      Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human colon carcinoma tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human stomach tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (2)

    发表研究结果有使用 ab2728?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2728 被引用在 2 文献中.

    • Yang MQ  et al. Interferon regulatory factor 1-Rab27a regulated extracellular vesicles promote liver ischemia/reperfusion injury. Hepatology 67:1056-1070 (2018). PubMed: 29059701
    • Zhu W  et al. Small GTPase ARF6 controls VEGFR2 trafficking and signaling in diabetic retinopathy. J Clin Invest 127:4569-4582 (2017). PubMed: 29058688

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