Anti-Cytohesin 2抗体[10A12] (ab2728)
Key features and details
- Mouse monoclonal [10A12] to Cytohesin 2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-Cytohesin 2抗体[10A12]
参阅全部 Cytohesin 2 一抗 -
描述
小鼠单克隆抗体[10A12] to Cytohesin 2 -
宿主
Mouse -
特异性
Detects recombinant human cytohesin-2 (ARNO). -
经测试应用
适用于: ICC/IF, IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein (His-tag) corresponding to Human Cytohesin 2.
Database link: Q99418 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯化说明
PEG purified IgG1 -
克隆
单克隆 -
克隆编号
10A12 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/20.
|
|
IHC-P |
1/25 - 1/200.
|
说明 |
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ICC/IF
1/20. |
IHC-P
1/25 - 1/200. |
靶标
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功能
Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. -
组织特异性
Ubiquitous. -
序列相似性
Contains 1 PH domain.
Contains 1 SEC7 domain. -
结构域
The PH domain is necessary and sufficient for plasma membrane relocalization. -
细胞定位
Cell membrane. Cytoplasm. Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. Requires also interaction with phosphoinositides for targeting to plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9266 Human
- Entrez Gene: 19158 Mouse
- Omim: 602488 Human
- SwissProt: Q99418 Human
- SwissProt: P63034 Mouse
- Unigene: 144011 Human
- Unigene: 272130 Mouse
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别名
- ARF exchange factor antibody
- ARF nucleotide binding site opener antibody
- ARF nucleotide-binding site opener antibody
see all
图片
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Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human colon carcinoma tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human stomach tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab2728 被引用在 3 文献中.
- Zhu X et al. Effect of Dual Endothelin Receptor Antagonist on a Retinal Degeneration Animal Model by Regulating Choroidal Microvascular Morphology. J Ophthalmol 2021:5688300 (2021). PubMed: 34840823
- Yang MQ et al. Interferon regulatory factor 1-Rab27a regulated extracellular vesicles promote liver ischemia/reperfusion injury. Hepatology 67:1056-1070 (2018). PubMed: 29059701
- Zhu W et al. Small GTPase ARF6 controls VEGFR2 trafficking and signaling in diabetic retinopathy. J Clin Invest 127:4569-4582 (2017). PubMed: 29058688