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Anti-Cytochrome P450 4A/CYP4A11抗体(ab3573)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (14)

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Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)

Key features and details

  • Rabbit polyclonal to Cytochrome P450 4A/CYP4A11
  • Suitable for: IHC-Fr, ICC/IF, IHC-P, WB, IP
  • Reacts with: Mouse, Rat, Rabbit, Hamster, Cat, Dog, Human, Pig
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Cytochrome P450 4A/CYP4A11抗体
    参阅全部 Cytochrome P450 4A/CYP4A11 一抗
  • 描述

    兔多克隆抗体to Cytochrome P450 4A/CYP4A11
  • 宿主

    Rabbit
  • 特异性

    The immunogen is homologous to rat cytochrome P450 4A2, 4A10, 4A12 and 4A14. Gene synonyms are Cyp4a11, Cyp4a1, Cyp4a8, and Cyp4a3, respectively. The specificity to these specific forms has not been confirmed experimentally. Please note nomenclature for specific forms may differ from mouse to rat.
  • 经测试应用

    适用于: IHC-Fr, ICC/IF, IHC-P, WB, IPmore details
  • 种属反应性

    与反应: Mouse, Rat, Rabbit, Hamster, Cat, Dog, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Rat Cytochrome P450 4A/CYP4A11 aa 400-500. The immunogen is homologous to rat cytochrome P450 4A2, A10, A12 and A14.
    Database link: P08516

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: Rat liver tissue lysate, H-4-II-E and HeLa cell lysate
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • Primary antibody说明

    The Cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA’s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, & 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic & brain microvessels.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3573于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/20 - 1/200.
IHC-P
1/100 - 1/500.
WB
1/200 - 1/2000. Predicted molecular weight: 59 kDa.
IP
Use at an assay dependent concentration.
说明
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/20 - 1/200.
IHC-P
1/100 - 1/500.
WB
1/200 - 1/2000. Predicted molecular weight: 59 kDa.
IP
Use at an assay dependent concentration.

靶标

  • 功能

    Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate, myristate and palmitate. Has little activity toward prostaglandins A1 and E1. Oxidizes arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE).
  • 组织特异性

    Kidney and liver.
  • 序列相似性

    Belongs to the cytochrome P450 family.
  • 细胞定位

    Endoplasmic reticulum membrane. Microsome membrane.
  • Target information above from: UniProt accession Q02928 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1579 Human
    • Entrez Gene: 24306 Rat
    • Entrez Gene: 298423 Rat
    • Entrez Gene: 50549 Rat
    • Omim: 601310 Human
    • SwissProt: Q02928 Human
    • SwissProt: P14581 Rabbit
    • SwissProt: P08516 Rat
    • SwissProt: P20816 Rat
    • SwissProt: P20817 Rat
    • Unigene: 1645 Human
    • Unigene: 726474 Human
    see all
  • 别名

    • 20-HETE synthase antibody
    • 20-hydroxyeicosatetraenoic acid synthase antibody
    • CP4AB_HUMAN antibody
    • CYP4A antibody
    • Cyp4a1 antibody
    • Cyp4a10 antibody
    • CYP4A11 antibody
    • Cyp4a14 antibody
    • Cyp4a3 antibody
    • CYP4A7 antibody
    • CYP4AII antibody
    • CYPIVA11 antibody
    • Cytochrome P-450HK-omega antibody
    • Cytochrome P450 4A1 antibody
    • Cytochrome P450 4A10 antibody
    • Cytochrome P450 4A11 antibody
    • Cytochrome P450 4A14 antibody
    • Cytochrome P450 4A2 antibody
    • Cytochrome P450 4A3 antibody
    • Cytochrome P450 4A7 antibody
    • Cytochrome P450HL-omega antibody
    • Fatty acid omega-hydroxylase antibody
    • Lauric acid omega-hydroxylase antibody
    see all

图片

  • Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
    Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
    All lanes : Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573) at 1/1000 dilution

    Lane 1 : Rat liver tissue lysate
    Lane 2 : H-4-II-E cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 59 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?



    Chemiluminescent detection

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)

    ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of H-4-II-E (rat) cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)

    ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm of Rat liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)

    ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of HeLa cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)

    ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of PC12 cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)

    ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm of Rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)

    ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm and membrane of Human liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

     

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (14)

发表研究结果有使用 ab3573?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3573 被引用在 14 文献中.

  • Cui W  et al. 20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1a pathway: A translational study. Cell Prolif 54:e12964 (2021). PubMed: 33314534
  • Han X  et al. 20-HETE synthesis inhibition promotes cerebral protection after intracerebral hemorrhage without inhibiting angiogenesis. J Cereb Blood Flow Metab 39:1531-1543 (2019). PubMed: 29485354
  • Lai YJ  et al. Estrogen receptor a promotes Cav1.2 ubiquitination and degradation in neuronal cells and in APP/PS1 mice. Aging Cell N/A:e12961 (2019). PubMed: 31012223
  • Li H  et al. Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction through AA Metabolism Modulated CYPs Pathway. Int J Biol Sci 15:2037-2050 (2019). PubMed: 31592228
  • Liu W  et al. CYP2C11 played a significant role in down-regulating rat blood pressure under the challenge of a high-salt diet. PeerJ 7:e6807 (2019). PubMed: 31065462
View all Publications for this product

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1-3 of 3 Abreviews or Q&A

Question

What do you recommend as positive and negative controls for these antibodies?

Read More

Abcam community

Verified customer

Asked on Nov 27 2006

Answer

Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and endothelial cells but not whole skin in the following paper: Leukotriene A4 hydrolase in human skin. J Invest Dermatol. 1994 Feb;102(2):253-7. PMID: 8106755 Our lysate ab30166 may be suitable as a negative control but I cannot guarantee you will not see some reactivity. For the cytochrome P450 enzymes, I suggest liver lysates. A positive control for ab4236 is human liver: ab29889. For ab13513 and ab3573, rat liver lysate ab29413 will be appropriate. I am reluctant to recommend a negative control since information regarding absence of expression is difficult to come by but since CP450 is associated with the ER, cytoplasmic fractions of liver lysates (which do not contain membrane) may be useful. For human liver, this would be ab29875, and for rat liver ab29409. You should also have a look at the references listed on the datasheets to see how other researchers have used the antibodies, though the references for the LTA4 are general and not specific to the antibody. I hope this helps. Please do not hesitate to contact us if you have any other questions.

Read More

Abcam Scientific Support

回复于 Nov 27 2006

Question

I am looking for an antibody against human Cytochrome P450 4A. In your products you have an antibody Rb anti rat CYP4A, which has not been tested against human. Is it possible for you to provide me with the amino acid sequence used for immunization, so I can allign rat and human sequence? -If they are very similar I would be interested in testing your antibody against human CYP4A.

Read More

Abcam community

Verified customer

Asked on Feb 20 2004

Answer

I think you must be interested in ab3573 -- Rabbit polyclonal to Cytochrome P450 4A. If so, all the information you need is on the online datasheet under the "immunogen section". Synthetic peptide D(431 PSRFAPDSPRHSHS(445). The immunizing peptide corresponds to amino acid residues 431-445 from rat cytochrome P450 4A. If this is not the antibody you are interested in, please let me know and I'll do my best to help you.

Read More

Abcam Scientific Support

回复于 Feb 20 2004

Question

Hello, I ran a Western blot and used your anti-Cytochrome P450 4A (ab3573). I also ran a positive control using your rat liver lysate (ab7943) with the anti-rabbit IgG (ab6721) for my secondary antibody. I attached a picture of the membrane with the molecular weight markers labeled in lane 6 and the postive control in lane 5. It looks as if I have 2 bands stained around the 50kDa area like I do in my samples (lane 4) as well. I was wondering if you had information as to which band was cytochrome P450 4A. Thanks for your help. Jennifer

Read More

Abcam community

Verified customer

Asked on Dec 12 2003

Answer

Thank you for your enquiry. Ab3573 detects an ~50 kDa protein representing P450 4A in rat liver extract and also detects, to a lesser extent, isoforms 4A1 and 4A3 (in the same rat liver extract sample, a slightly larger band representing 4A3 is also clearly visible). Thus, on your gel, the smaller band would be cytochrome P450 4A. If you have any more questions, please contact us again.

Read More

Abcam Scientific Support

回复于 Dec 15 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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