重组Anti-Cytochrome P450 3A4/CYP3A4抗体[EPR6202] (ab124921)
![Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)](https://www.abcam.cn/ps/products/124/ab124921/Images/ab124921-347182-anti-cytochrome-p450-3a4cyp3a4-antibody-epr6202-westernblot-human.jpg "Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6202] to Cytochrome P450 3A4/CYP3A4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cytochrome P450 3A4/CYP3A4抗体[EPR6202]
参阅全部 Cytochrome P450 3A4/CYP3A4 一抗 -
描述
兔单克隆抗体[EPR6202] to Cytochrome P450 3A4/CYP3A4 -
宿主
Rabbit -
特异性
Cross-reactivity with other CYP3A family: ab124921 has high possibility of reacting with CYP3A7 based on our internal test.
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经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Cytochrome P450 3A4/CYP3A4 aa 450-550 (C terminal). The exact sequence is proprietary.
(Peptide available asab234892) -
阳性对照
- WB: Human fetal and liver cell lysates IHC-P: Human liver tissue IP: Human liver cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解离常数（KD）
KD = 2.63 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6202 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124921于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 57 kDa.
For unpurified use at 1/10000 - 1/50000 dilution. |
| IP |
1/10 - 1/100.
|
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| IHC-P |
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/100 - 1/250 dilution. |
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 57 kDa. For unpurified use at 1/10000 - 1/50000 dilution. |
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IP
1/10 - 1/100. |
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IHC-P
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250 dilution. |
靶标
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功能
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide. -
组织特异性
Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level). -
序列相似性
Belongs to the cytochrome P450 family. -
翻译后修饰
Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro). -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1576 Human
- Omim: 124010 Human
- SwissProt: P08684 Human
- Unigene: 728751 Human
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别名
- 1,8-cineole 2-exo-monooxygenase antibody
- Albendazole monooxygenase antibody
- Albendazole sulfoxidase antibody
see all
图片
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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921) at 1/1000 dilution (Purified)
Lane 1 : Human liver lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell), Whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?This antibody fails to detect BOI in normal HepG2 cells which is positive reported by PMID 26089940 and 25141173
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![Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)](https://www.abcam.cn/ps/products/124/ab124921/Images/ab124921-138928-anti-cytochrome-p450-3a4-antibody-epr6202-western-blot.jpg)
Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921) at 1/10000 dilution ((unpurified)) + Human fetal liver lysate at 10 µg
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 57 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)](https://www.abcam.cn/ps/products/124/ab124921/Images/ab124921-6-anti-cytochrome-p450-3a4cyp3a4-antibody-epr6202-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Cytochrome P450 3A4/CYP3A4 with purified ab124921 at 1/400 dilution (0.35 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunoprecipitation - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)](https://www.abcam.cn/ps/products/124/ab124921/Images/ab124921-7-anti-cytochrome-p450-3a4cyp3a4-antibody-epr6202-immunoprecipitation-liver-human.jpg)
Immunoprecipitation - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)ab124921 (purified) at 1/20 dilution (1ug) immunoprecipitating Cytochrome P450 3A4/CYP3A4 in Human liver lysates.
Lane 1: Human liver lysates 10ug
Lane 2 (+): ab124921 & Human liver lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124921 in Human liver lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)](https://www.abcam.cn/ps/products/124/ab124921/Images/ab124921-172966-anti-cytochrome-p450-3a4-antibody-epr6202-western-blot.jpg)
Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)HepG2 cells were incubated at 37°C for 48h with vehicle control (0 μM) and different concentrations of nicardipine hydrochloride (ab120531) in DMSO. Increased expression of Cytochrome P450 3A4/CYP3A4 (ab124921, unpurified) correlates with an increase in nicardipine hydrochloride concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab124921 (unpurified) at 1/10000 dilution and ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)](https://www.abcam.cn/ps/products/124/ab124921/Images/ab124921-138927-anti-cytochrome-p450-3a4-antibody-epr6202-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)ab124921 (unpurified), at a 1/100 dilution, staining Cytochrome P450 3A4/CYP3A4 in paraffin embedded Human liver tissue by Immunohistochemistry.
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![OI-RD Scanning - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)](https://www.abcam.cn/ps/products/124/ab124921/Images/ab124921-138926-anti-cytochrome-p450-3a4-antibody-epr6202.jpg)
OI-RD Scanning - Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921) -
![Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)](https://www.abcam.cn/ps/products/124/ab124921/Images/ab124921-9-benefits-of-recombinant-antibodies.png)
Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab124921 被引用在 13 文献中.
- Wang X et al. Long Noncoding RNAs Hepatocyte Nuclear Factor 4A Antisense RNA 1 and Hepatocyte Nuclear Factor 1A Antisense RNA 1 Are Involved in Ritonavir-Induced Cytotoxicity in Hepatoma Cells. Drug Metab Dispos 50:704-715 (2022). PubMed: 34949673
- Zhang XB et al. Intermittent Hypoxia Inhibits Hepatic CYP1a2 Expression and Delays Aminophylline Metabolism. Evid Based Complement Alternat Med 2022:2782702 (2022). PubMed: 35529917
- Florentino RM et al. Biofabrication of synthetic human liver tissue with advanced programmable functions. iScience 25:105503 (2022). PubMed: 36404924
- Prawira A et al. Ribociclib enhances infigratinib-induced cancer cell differentiation and delays resistance in FGFR-driven hepatocellular carcinoma. Liver Int 41:608-620 (2021). PubMed: 33179425
- Wang X et al. DNA methylation determines the regulation of pregnane X receptor on CYP3A4 expression. Clin Exp Pharmacol Physiol 48:250-259 (2021). PubMed: 33048369