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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cytochromes

Anti-Cytochrome P450 3A4/CYP3A4抗体(ab3572)

  • Datasheet
  • SDS
Submit a review Q&A (7)References (23)

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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
  • Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
  • Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
  • Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)

Key features and details

  • Rabbit polyclonal to Cytochrome P450 3A4/CYP3A4
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Cytochrome P450 3A4/CYP3A4抗体
    参阅全部 Cytochrome P450 3A4/CYP3A4 一抗
  • 描述

    兔多克隆抗体to Cytochrome P450 3A4/CYP3A4
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein (His-tag) corresponding to Human Cytochrome P450 3A4/CYP3A4. Purified, His-tagged, full length human P450 3A4 fusion protein.

  • 阳性对照

    • WB: Rat and mouse liver lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Ammonium Sulphate Precipitation
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Cytochrome P450 3A4/CYP3A4 protein (ab114328)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3572于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).

A 46kDa band is detected in rat liver extracts.

说明
WB
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).

A 46kDa band is detected in rat liver extracts.

靶标

  • 功能

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
  • 组织特异性

    Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
  • 序列相似性

    Belongs to the cytochrome P450 family.
  • 翻译后修饰

    Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
  • 细胞定位

    Endoplasmic reticulum membrane. Microsome membrane.
  • Target information above from: UniProt accession P08684 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1576 Human
    • Omim: 124010 Human
    • SwissProt: P08684 Human
    • Unigene: 728751 Human
    • 别名

      • 1,8-cineole 2-exo-monooxygenase antibody
      • Albendazole monooxygenase antibody
      • Albendazole sulfoxidase antibody
      • CP33 antibody
      • CP34 antibody
      • CP3A4_HUMAN antibody
      • CYP3 antibody
      • CYP3A antibody
      • CYP3A3 antibody
      • CYP3A4 antibody
      • CYPIIIA3 antibody
      • CYPIIIA4 antibody
      • Cytochrome P450 3A3 antibody
      • Cytochrome P450 3A4 antibody
      • Cytochrome P450 family 3 subfamily A polypeptide 4 antibody
      • Cytochrome P450 HLp antibody
      • Cytochrome P450 NF-25 antibody
      • Cytochrome P450 subfamily IIIA polypeptide 4 antibody
      • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3 antibody
      • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 antibody
      • Cytochrome P450-PCN1 antibody
      • Glucocorticoid inducible P450 antibody
      • HLP antibody
      • MGC126680 antibody
      • NF 25 antibody
      • NF25 antibody
      • Nifedipine oxidase antibody
      • P450 III steroid inducible antibody
      • P450 PCN1 antibody
      • P450, family III antibody
      • P450C3 antibody
      • P450PCN1 antibody
      • Quinine 3 monooxygenase antibody
      • Quinine 3-monooxygenase antibody
      • Taurochenodeoxycholate 6 alpha hydroxylase antibody
      • Taurochenodeoxycholate 6-alpha-hydroxylase antibody
      see all

    图片

    • Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
      Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
      All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572) at 1/2000 dilution

      Lane 1 : Mouse liver tissue extract
      Lane 2 : Rat liver tissue extract
      Lane 3 : Mouse stomach tissue extract
      Lane 4 : Rat stomach tissue extract

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

      Developed using the ECL technique.

      Predicted band size: 57 kDa
      Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.



      Electrophoresed on 4-12% BisTris gel transferred onto a nitrocellulose membrane

    • Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
      Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
      All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572) at 1/2000 dilution

      Lane 1 : Mouse liver with Skimmed milk
      Lane 2 : Rat liver with Skimmed milk

      Lysates/proteins at 30 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-Rabbit IgG (H+L) at 1/5000 dilution

      Predicted band size: 57 kDa

    • Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
      Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)

      Western blot of Cytochrome P450 3A4/CYP3A4 on rat liver extract using ab3572.

    • Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)
      Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)

      ab3572 at a dilution of 1/2000 staining Cytochrome P450 3A4/CYP3A4 in rat liver extract by Western blot.

    实验方案

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (23)

    发表研究结果有使用 ab3572?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3572 被引用在 23 文献中.

    • Liu J  et al. Disrupted hepatic pentose phosphate pathway directly participates in and indirectly promotes CYP3A reduction: A new strategy for CYP3A-mediated drug hepatotoxicity. Br J Pharmacol 177:1538-1555 (2020). PubMed: 31670839
    • Hu N  et al. P-glycoprotein associated with diabetes mellitus and survival of patients with pancreatic cancer: 8-year follow-up. Braz J Med Biol Res 53:e10068 (2020). PubMed: 33053111
    • Reed L  et al. Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine. Toxicol Appl Pharmacol 366:64-74 (2019). PubMed: 30685480
    • Zhou XY  et al. Enzymatic Activities of CYP3A4 Allelic Variants on Quinine 3-Hydroxylation In Vitro. Front Pharmacol 10:591 (2019). PubMed: 31214030
    • Katsuda T  et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. Elife 8:N/A (2019). PubMed: 31393263
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Question

    Hi, I have a question about ab3572. I performed western blot with it and got two bands which have similar size. I suppose that these are CYP3A4 and 3A5. There was no comment about cross-reactivity with CYP3A5 in the datasheet. Does ab3572 have crosse-reactivity with CYP3A5? Even if there's no data about cross-reactivity, can we expect it? I am looking forward to your kind reply. Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 12 2009

    Answer

    Thank you for your enquiry. I can confirm that this product detects cytochrome P450 3A4 from human and rat tissues and cells. Unfortunately, we do not have any information or data showing cross reactivity with 3A5 but it may detect, to a lesser extent, other P450 isoforms. I am sorry there is not much information on the matter but you have any other questions, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Feb 12 2009

    Question

    What is the concentration of the current lot of ab3572?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 25 2005

    Answer

    I was informed by the originator of the antibody that the concentration was not determined, quite surprisingly. They predict the concentration to be around 1mg/ml. I'm sorry I couldn't help you more. Please do not hesitate to contact me again if I can be of further assistance,

    Read More

    Abcam Scientific Support

    回复于 Jul 25 2005

    Question

    Customer has said that there is no 3A4 present in rat - do you have any information regarding this?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 21 2004

    Answer

    It is true that rats do not express 3A4. This is a human specific CYP. Rats do express others in the CYP family which this antibody cross-reacts with since the immunogen was full length human P450 3A4 fusion protein. That explains the cross-reaction with other rat CYP families (only we don't know which exact one!). If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Sep 24 2004

    Question

    1. more bands 2. rat liver whole cell lysates 3. 50 micro grams 4. i used three different protease inhibitors and used sonication 5. no i didnt heat the samples 6. primary antibody raised in rabbit 7. 1 in 2000 8. incubated over night. did five to six washes 9. the secondary antibody concentrations were tested from 1 in 2000 to 1 in 10,000. 10. no it is not due to the secondary antibody as they look were no bands in secondary antibody control 11. incubated for one hour in secondary antibody 12. we are using horse raddish peroxidase and taking the pictures in x ray film 13. yes i did a column as control in which i didnt add the primary antibody 14. i tried different concentrations of secondary

    Read More

    Abcam community

    Verified customer

    Asked on Jul 14 2004

    Answer

    Thank you very much for the details that you have provided and for your patience. What was the lot number that you received? Why did you not heat the samples to denature the proteins? In addition to the non-specific bands that you are seeing, did you see the expected band at 46 kDa? I can offer you a replacement vial or a refund, please send me the Abcam order number of purchase order number that was used.

    Read More

    Abcam Scientific Support

    回复于 Jul 21 2004

    Question

    Customer would like to know the concentration of the batch currently in stock.

    Read More

    Abcam community

    Verified customer

    Asked on May 18 2004

    Answer

    Thank you for your enquiry. We did not perform experiments to determine the approximate concentration for these lots. It is purified IgG, and we don't know how much of it is specific for Cytochrome P450 3A4. We would recommend using this antibody at 1/2000 dilution for Western blot application.

    Read More

    Abcam Scientific Support

    回复于 May 19 2004

    Question

    I am working on fescue toxin in rats liver. I am plannig for immuno histo chemistry in around 72 samples. I was strongly suggested to use the antibodies from ur company. so i went to abcam website. in that i saw that this antibody is tested only for WB. so i am not sure if i can use this for IHC. so i request you to send me a very small quantity of the CYTOCHROME P 450 3A4 (AB3572) sample. so that i can test run for a couple of samples.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2003

    Answer

    Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Nov 21 2003

    Question

    I am writing to you with reference to your antibody to Cytochrome P450 3A4 (ab3572). I need to screen quite large numbers of transgenic cyp3A4 regulatable cells that I am developing and consequently will need an antibody to cyp3A4. Before I purchase the antibody, I will need to know if it is applicable to my research use. Therefore would it be possible to send me a very small quantity (5 or 10microL) of your anti cyp3A4 for me to test prior to purchase? Many thanks for your time.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. This antibody has been tested in Western Blotting and cross reacts with human and rat. If you plan to use the antibody in an application other than Western Blotting, please let us know and I will look into offering you a discount on your first vial.

    Read More

    Asdf Edo

    Abcam Scientific Support

    回复于 Oct 13 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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