Anti-Cytochrome P450 3A4/CYP3A4抗体(ab3572)
Key features and details
- Rabbit polyclonal to Cytochrome P450 3A4/CYP3A4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cytochrome P450 3A4/CYP3A4抗体
参阅全部 Cytochrome P450 3A4/CYP3A4 一抗 -
描述
兔多克隆抗体to Cytochrome P450 3A4/CYP3A4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein (His-tag) corresponding to Human Cytochrome P450 3A4/CYP3A4. Purified, His-tagged, full length human P450 3A4 fusion protein.
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阳性对照
- WB: Rat and mouse liver lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).
A 46kDa band is detected in rat liver extracts. |
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa). A 46kDa band is detected in rat liver extracts. |
靶标
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功能
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide. -
组织特异性
Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level). -
序列相似性
Belongs to the cytochrome P450 family. -
翻译后修饰
Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro). -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1576 Human
- Omim: 124010 Human
- SwissProt: P08684 Human
- Unigene: 728751 Human
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别名
- 1,8-cineole 2-exo-monooxygenase antibody
- Albendazole monooxygenase antibody
- Albendazole sulfoxidase antibody
see all
图片
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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572) at 1/2000 dilution
Lane 1 : Mouse liver tissue extract
Lane 2 : Rat liver tissue extract
Lane 3 : Mouse stomach tissue extract
Lane 4 : Rat stomach tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.Electrophoresed on 4-12% BisTris gel transferred onto a nitrocellulose membrane
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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572) at 1/2000 dilution
Lane 1 : Mouse liver with Skimmed milk
Lane 2 : Rat liver with Skimmed milk
Lysates/proteins at 30 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 57 kDa -
Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)Western blot of Cytochrome P450 3A4/CYP3A4 on rat liver extract using ab3572.
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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)ab3572 at a dilution of 1/2000 staining Cytochrome P450 3A4/CYP3A4 in rat liver extract by Western blot.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab3572 被引用在 23 文献中.
- Liu J et al. Disrupted hepatic pentose phosphate pathway directly participates in and indirectly promotes CYP3A reduction: A new strategy for CYP3A-mediated drug hepatotoxicity. Br J Pharmacol 177:1538-1555 (2020). PubMed: 31670839
- Hu N et al. P-glycoprotein associated with diabetes mellitus and survival of patients with pancreatic cancer: 8-year follow-up. Braz J Med Biol Res 53:e10068 (2020). PubMed: 33053111
- Reed L et al. Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine. Toxicol Appl Pharmacol 366:64-74 (2019). PubMed: 30685480
- Zhou XY et al. Enzymatic Activities of CYP3A4 Allelic Variants on Quinine 3-Hydroxylation In Vitro. Front Pharmacol 10:591 (2019). PubMed: 31214030
- Katsuda T et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. Elife 8:N/A (2019). PubMed: 31393263