Anti-Cytochrome P450 2E1抗体(ab28146)
Key features and details
- Rabbit polyclonal to Cytochrome P450 2E1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
概述
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产品名称
Anti-Cytochrome P450 2E1抗体
参阅全部 Cytochrome P450 2E1 一抗 -
描述
兔多克隆抗体to Cytochrome P450 2E1 -
宿主
Rabbit -
特异性
The antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Rabbit, Human -
免疫原
Full length native protein (purified) (Rat)
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常规说明
The antibody has been shown to inhibit microsomal benzene metabolism
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
Primary antibody说明
The antibody has been shown to inhibit microsomal benzene metabolism -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28146于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.
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| ICC/IF |
1/1000.
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| 说明 |
|---|
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WB
1/5000. Predicted molecular weight: 50-55 kDa. This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody. |
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ICC/IF
1/1000. |
靶标
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功能
Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1571 Human
- Entrez Gene: 13106 Mouse
- Entrez Gene: 25086 Rat
- Omim: 124040 Human
- SwissProt: P05181 Human
- SwissProt: Q05421 Mouse
- SwissProt: P08682 Rabbit
- SwissProt: P05182 Rat
see all -
别名
- 4-nitrophenol 2-hydroxylase antibody
- CP2E1_HUMAN antibody
- CPE1 antibody
see all
图片
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Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human liver microsomes
Lane 3 : Cell lysates prepared from rat liver microsomes
Lane 4 : Cell lysates prepared from mouse liver microsomes
Lane 5 : Cell lysates prepared from rabbit liver microsomes
Predicted band size: 50-55 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 2E1 antibody (ab28146)ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146)Image courtesy of an anonymous Abreview.All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution
All lanes : Tissue lysate prepared from normal murine liver
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 50-55 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (153)
ab28146 被引用在 153 文献中.
- Fortin CL et al. Temporal Dynamics of Metabolic Acquisition in Grafted Engineered Human Liver Tissue. Adv Biol (Weinh) 7:e2200208 (2023). PubMed: 36328790
- Yu D et al. Oridonin ameliorates acetaminophen-induced acute liver injury through ATF4/PGC-1α pathway. Drug Dev Res 84:211-225 (2023). PubMed: 36567664
- Jia H et al. The role of altered lipid composition and distribution in liver fibrosis revealed by multimodal nonlinear optical microscopy. Sci Adv 9:eabq2937 (2023). PubMed: 36638165
- Pu W et al. Bipotent transitional liver progenitor cells contribute to liver regeneration. Nat Genet 55:651-664 (2023). PubMed: 36914834
- Hu G et al. Identification of Cytochrome P450 2E1 as a Novel Target in Glioma and Development of Its Inhibitor as an Anti-Tumor Agent. Adv Sci (Weinh) 10:e2301096 (2023). PubMed: 37283464