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Anti-Cytochrome P450 2B1 + 2B2抗体[b/e4 (9.20)] (ab22721)

Reviews (2)Q&A (1)References (1)

Key features and details

  • Mouse monoclonal [b/e4 (9.20)] to Cytochrome P450 2B1 + 2B2
  • Suitable for: ELISA, WB, IP
  • Reacts with: Mouse, Rat
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Cytochrome P450 2B1 + 2B2抗体[b/e4 (9.20)]
    参阅全部 Cytochrome P450 2B1 + 2B2 一抗

  • 描述

    小鼠单克隆抗体[b/e4 (9.20)] to Cytochrome P450 2B1 + 2B2

  • 宿主

    Mouse

  • 特异性

    Clone b/e4 does not react with CYP 1A1, 1A2, 2C6, 2C7, 2C11, 4A2, 4A3.

  • 经测试应用

    适用于: ELISA, WB, IPmore details

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Full length protein (Rat).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab22721于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP
Use at an assay dependent concentration.

靶标

  • 相关性

    The Cytochrome P450 superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

  • 细胞定位

    Membrane bound, endoplasmic reticulum

  • 数据库链接

    • 别名

      • Cyp2b 1 antibody
      • Cyp2b 2 antibody
      • Cyp2b1 antibody
      • Cyp2b2 antibody
      • Cypbe antibody
      • Cype antibody
      • CYPIIB1 antibody
      • CYPIIB2 antibody
      • Cytochrome P 450b antibody
      • Cytochrome P 450b type e antibody
      • Cytochrome P 450e M antibody
      • Cytochrome P450 2B1 antibody
      • Cytochrome P450 2B2 antibody
      • Cytochrome P450 family 2 subfamily b polypeptide 1 antibody
      • Cytochrome P450 family 2 subfamily b polypeptide 2 antibody
      • Cytochrome P450 subfamily IIB phenobarbital inducible b e antibody
      • ec 1.14.14.1 antibody
      • P450 B antibody
      • P450 LM2 antibody
      • P450 PB1 and P450 PB2 antibody
      • P450 PB4 antibody
      • P450b antibody
      • P450E antibody
      see all

    文献 (1)

    发表研究结果有使用 ab22721？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab22721 被引用在 1 文献中.

    • Probert PM  et al. Utility of B-13 progenitor-derived hepatocytes in hepatotoxicity and genotoxicity studies. Toxicol Sci 137:350-70 (2014). WB ; Rat . PubMed: 24235770

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e4 (9.20)]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Other
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Hydrogen peroxide, (HISTOLS-Peroxidase Inhibitor, Histopathology Ltd., Pecs, Hungary) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 30 2017

    Western blot abreview for Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e4 (9.20)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Tissue lysate - whole (Male rat liver)
    Specification
    Male rat liver
    Treatment
    100mg/kg phenobarbital for 24hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 14 2013

    Question

    Dear Abcam representative,
    I am looking for suitable anti-CYP2B1 and anti-CYP2E1 antibodies (reactivity to rats) for my research purpose. Iam interested toyour products (cat.# ab 22721 and ab28146) for these two enzymes. Would you mindproviding me some information about any lab has already used these products or any article that has mentioned these products? Actually I need some supporting citations if I want topurchase these particularproducts.

    Thank you.
    Sincerely,

    Read More
    Answer

    Thank you for contacting us.

    Both mentioned antibodies have been validated to react with rat species. At Abcam, our aim is to share all the information we have available from our products in the Website, to help customers decide the best option for their requirements.

    For product ab28146 there are nine references available (under the Tab Specific References) as well as three Abreviews corresponding to customer’s results using this antibody. I hope you find them useful.

    Unfortunately, there aren’t any references or Abreviews available for ab22721. As soon as we gather further information or publications referring to our products, we will immediately update our datasheets.

    In any case, all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

    To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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