Anti-Cytochrome P450 1A2抗体[3B8C1] (ab56073)
![Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)](https://www.abcam.cn/ps/products/56/ab56073/Images/ab56073-69410-anti-cytochrome-p450-1a2-antibody-3b8c1-western-blot.jpg "Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)")
Key features and details
- Mouse monoclonal [3B8C1] to Cytochrome P450 1A2
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-Cytochrome P450 1A2抗体[3B8C1]
参阅全部 Cytochrome P450 1A2 一抗 -
描述
小鼠单克隆抗体[3B8C1] to Cytochrome P450 1A2 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Rat Cytochrome P450 1A2 proteins
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阳性对照
- IHC-P: Normal human liver sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
3B8C1 -
骨髓瘤
Ag8.653 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab56073于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
|
|
| IHC-P |
Use a concentration of 1 - 5 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
|
IHC-P
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. -
组织特异性
Liver. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1544 Human
- Omim: 124060 Human
- SwissProt: P05177 Human
- Unigene: 1361 Human
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别名
- Aryl hydrocarbon hydroxylase antibody
- CP 12 antibody
- CP12 antibody
see all
图片
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Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)All lanes : Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)
Lane 2 : recombinant CYP1B1 at 0.5pmol/lane
Lane 3 : recombinant CYP1A1 at 0.5pmol/lane
Lane 4 : recombinant CYP1A2 at 0.5pmol/lane
Predicted band size: 58 kDa
Observed band size: 58 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)](https://www.abcam.cn/ps/products/56/ab56073/Images/ab56073-69409-anti-cytochrome-p450-1a2-antibody-3b8c1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)Immunohistochemical staining of formalin-fixed, paraffin-embedded normal human liver tissue section using ab56073.
数据表及文件
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Datasheet download
文献 (6)
ab56073 被引用在 6 文献中.
- Saha A et al. Identification of the contact region responsible for the formation of the homomeric CYP1A2•CYP1A2 complex. Biochem J 478:2163-2178 (2021). PubMed: 34032264
- Zhang X et al. A tryptophan derivative, ITE, enhances liver cell metabolic functions in vitro. Int J Mol Med 39:101-112 (2017). PubMed: 27959388
- Higuchi Y et al. Functional polymer-dependent 3D culture accelerates the differentiation of HepaRG cells into mature hepatocytes. Hepatol Res 46:1045-57 (2016). PubMed: 26724677
- Gustafsson F et al. A correlation between the in vitro drug toxicity of drugs to cell lines that express human P450s and their propensity to cause liver injury in humans. Toxicol Sci 137:189-211 (2014). WB ; Human . PubMed: 24085192
- Fan J et al. Up-regulation of transporters and enzymes by the vitamin D receptor ligands, 1alpha,25-dihydroxyvitamin D3 and vitamin D analogs, in the Caco-2 cell monolayer. J Pharmacol Exp Ther 330:389-402 (2009). PubMed: 19414624
- Fisher CD et al. Hepatic cytochrome P450 enzyme alterations in humans with progressive stages of nonalcoholic fatty liver disease. Drug Metab Dispos 37:2087-94 (2009). PubMed: 19651758
