重组Anti-Cytochrome P450 17A1/CYP17A1抗体[EPR6293] (ab125022)
![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)](https://www.abcam.cn/ps/products/125/ab125022/Images/ab125022-318900-anti-cytochrome-p450-17a1-antibody-epr6293-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1
- Suitable for: WB, IP, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cytochrome P450 17A1/CYP17A1抗体[EPR6293]
参阅全部 Cytochrome P450 17A1/CYP17A1 一抗 -
描述
兔单克隆抗体[EPR6293] to Cytochrome P450 17A1/CYP17A1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IF, Flow Cyt (Intra)more details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Cytochrome P450 17A1/CYP17A1 aa 100-200. The exact sequence is proprietary.
Database link: P05093 -
阳性对照
- WB: Human adrenal gland, mouse and rat heart lysates, human fetal heart lysate, SK-OV-3, HeLa and Jurkat lysates; Flow Cyt (intra): HeLa cells ICC/IF: HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6293 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 647 Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab203743)
- Alexa Fluor® 488 Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab203995)
- HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab204022)
- PE Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab223958)
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] - Low endotoxin, Azide free (ab226009)
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] - BSA and Azide free (ab271888)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125022于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
|
| IP |
1/10 - 1/100.
|
|
| ICC/IF | (1) |
1/100 - 1/250.
|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa). |
|
IP
1/10 - 1/100. |
|
ICC/IF
1/100 - 1/250. |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. -
通路
Lipid metabolism; steroid biosynthesis. -
疾病相关
Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic). -
序列相似性
Belongs to the cytochrome P450 family. -
翻译后修饰
Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1586 Human
- Omim: 609300 Human
- SwissProt: P05093 Human
- Unigene: 438016 Human
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别名
- 20 lyase antibody
- CP17A_HUMAN antibody
- CPT7 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 17A1/CYP17A1 with Purified ab125022 at 1:100 dilution (3.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)](https://www.abcam.cn/ps/products/125/ab125022/Images/ab125022-318916-anti-cytochrome-p450-17a1-antibody-epr6293-western-blot.jpg)
Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/2000 dilution (purified) + Human fetal heart lysates at 15 µg
Predicted band size: 57 kDa -
![Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)](https://www.abcam.cn/ps/products/125/ab125022/Images/ab125022-318908-anti-cytochrome-p450-17a1-antibody-epr6293-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)ab125022 (purified) at 1:30 dilution (2µg) immunoprecipitating Cytochrome P450 17A1/CYP17A1 in Human fetal heart lysate.
Lane 1 (input): Human fetal heart lysate 10µg
Lane 2 (+): ab125022 & Human fetal heart lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125022 in Human fetal heart lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)](https://www.abcam.cn/ps/products/125/ab125022/Images/ab125022-139153-anti-cytochrome-p450-17a1-antibody-epr6293-western-blot.jpg)
Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/1000 dilution
Lane 1 : Human adrenal gland lysate
Lane 2 : SK-OV-3 lysate
Lane 3 : HeLa lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)](https://www.abcam.cn/ps/products/125/ab125022/Images/ab125022-270662-anti-cytochrome-p450-17a1-antibody-epr6293-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytochrome P450 17A1/CYP17A1 with purified ab125022 at 1/220 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)](https://www.abcam.cn/ps/products/125/ab125022/Images/ab125022-8-benefits-of-recombinant-antibodies.png)
Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (34)
ab125022 被引用在 34 文献中.
- Shen Y et al. New Target for Minoxidil in the Treatment of Androgenetic Alopecia. Drug Des Devel Ther 17:2537-2547 (2023). PubMed: 37645625
- Choi SR et al. Phase-specific differential regulation of mechanical allodynia in a murine model of neuropathic pain by progesterone. Front Pharmacol 14:1253901 (2023). PubMed: 38152690
- Zhang Y et al. Zika Virus Infection in the Ovary Induces a Continuously Elevated Progesterone Level and Compromises Conception in Interferon Alpha/Beta Receptor-Deficient Mice. J Virol 96:e0118921 (2022). PubMed: 34730391
- Gudelska M et al. Chemerin Affects P4 and E2 Synthesis in the Porcine Endometrium during Early Pregnancy. Int J Mol Sci 23:N/A (2022). PubMed: 35055130
- Luo Q et al. hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms. Reprod Biol Endocrinol 20:125 (2022). PubMed: 35986315