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重组RabMAb

重组Anti-Cytochrome P450 17A1/CYP17A1抗体[EPR6293] (ab125022)

Reviews (3) Submit a question References (34)
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
  • Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
  • Flow Cytometry (Intracellular) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
  • Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1
  • Suitable for: WB, IP, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP PE

概述

  • 产品名称

    Anti-Cytochrome P450 17A1/CYP17A1抗体[EPR6293]
    参阅全部 Cytochrome P450 17A1/CYP17A1 一抗

  • 描述

    兔单克隆抗体[EPR6293] to Cytochrome P450 17A1/CYP17A1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, ICC/IF, Flow Cyt (Intra)more details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human Cytochrome P450 17A1/CYP17A1 aa 100-200. The exact sequence is proprietary.
    Database link: P05093

  • 阳性对照

    • WB: Human adrenal gland, mouse and rat heart lysates, human fetal heart lysate, SK-OV-3, HeLa and Jurkat lysates; Flow Cyt (intra): HeLa cells ICC/IF: HeLa cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab125022于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
IP
1/10 - 1/100.
ICC/IF (1)
1/100 - 1/250.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
IP
1/10 - 1/100.
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

  • 通路

    Lipid metabolism; steroid biosynthesis.

  • 疾病相关

    Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).

  • 序列相似性

    Belongs to the cytochrome P450 family.

  • 翻译后修饰

    Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 20 lyase antibody
      • CP17A_HUMAN antibody
      • CPT7 antibody
      • CYP17 antibody
      • CYP17A1 antibody
      • CYPXVII antibody
      • Cytochrome P450 17A1 antibody
      • Cytochrome P450 family 17 antibody
      • Cytochrome P450 family 17 subfamily A polypeptide 1 antibody
      • Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia antibody
      • Cytochrome p450 XVIIA1 antibody
      • Cytochrome P450-C17 antibody
      • Cytochrome P450c17 antibody
      • OTTHUMP00000020382 antibody
      • P450 C17 antibody
      • P450c17 antibody
      • S17AH antibody
      • Steroid 17 alpha hydroxylase/17,20 lyase antibody
      • Steroid 17 alpha monooxygenase antibody
      • Steroid 17-alpha-hydroxylase/17 antibody
      • Steroid 17-alpha-monooxygenase antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 17A1/CYP17A1 with Purified ab125022 at 1:100 dilution (3.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
      Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
      Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/2000 dilution (purified) + Human fetal heart lysates at 15 µg

      Predicted band size: 57 kDa

    • Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
      Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

      ab125022 (purified) at 1:30 dilution (2µg) immunoprecipitating Cytochrome P450 17A1/CYP17A1 in Human fetal heart lysate.
      Lane 1 (input): Human fetal heart lysate 10µg
      Lane 2 (+): ab125022 & Human fetal heart lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125022 in Human fetal heart lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
      Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
      All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/1000 dilution

      Lane 1 : Human adrenal gland lysate
      Lane 2 : SK-OV-3 lysate
      Lane 3 : HeLa lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?

    • Flow Cytometry (Intracellular) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
      Flow Cytometry (Intracellular) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytochrome P450 17A1/CYP17A1 with purified ab125022 at 1/220 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

       

       

    • Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
      Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

    文献 (34)

    发表研究结果有使用 ab125022？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab125022 被引用在 34 文献中.

    • Shen Y  et al. New Target for Minoxidil in the Treatment of Androgenetic Alopecia. Drug Des Devel Ther 17:2537-2547 (2023). PubMed: 37645625
    • Choi SR  et al. Phase-specific differential regulation of mechanical allodynia in a murine model of neuropathic pain by progesterone. Front Pharmacol 14:1253901 (2023). PubMed: 38152690
    • Zhang Y  et al. Zika Virus Infection in the Ovary Induces a Continuously Elevated Progesterone Level and Compromises Conception in Interferon Alpha/Beta Receptor-Deficient Mice. J Virol 96:e0118921 (2022). PubMed: 34730391
    • Gudelska M  et al. Chemerin Affects P4 and E2 Synthesis in the Porcine Endometrium during Early Pregnancy. Int J Mol Sci 23:N/A (2022). PubMed: 35055130
    • Luo Q  et al. hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms. Reprod Biol Endocrinol 20:125 (2022). PubMed: 35986315
    View all Publications for this product

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]

     Excellent
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution low pH
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Prostate
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    提交于 Jun 25 2018

    Western blot abreview for Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Prostate Cancer Cell Lines)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Treatment
    Hormone treatment
    Specification
    Prostate Cancer Cell Lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. XXXXXX XXXXXX

    Verified customer

    提交于 Sep 27 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (H1299 Lung cancer cell line)
    Permeabilization
    Yes - 0.1% v/v TritonX-100
    Specification
    H1299 Lung cancer cell line
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    提交于 May 31 2016

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