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Anti-Cytochrome C Oxidase subunit VIc/COX6C抗体[3G5F7G3] (ab110267)

Submit a review Q&A (1)References (8)
Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
  • Flow Cytometry - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)

Key features and details

  • Mouse monoclonal [3G5F7G3] to Cytochrome C Oxidase subunit VIc/COX6C
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Rat, Cow, Human
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488

概述

  • 产品名称

    Anti-Cytochrome C Oxidase subunit VIc/COX6C抗体[3G5F7G3]
    参阅全部 Cytochrome C Oxidase subunit VIc/COX6C 一抗

  • 描述

    小鼠单克隆抗体[3G5F7G3] to Cytochrome C Oxidase subunit VIc/COX6C

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, Flow Cyt, WBmore details

  • 种属反应性

    与反应: Rat, Cow, Human

  • 免疫原

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Isolated mitochondria from Human heart, bovine heart, and rat heart. IF/ICC: HeLa cell line

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110267于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 9 kDa.
说明
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 9 kDa.

靶标

图片

  • Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
    Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
    All lanes : Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267) at 2 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 50 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg

    Predicted band size: 9 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)

    ICC/IF image of ab110267 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110267, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
    Flow Cytometry - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
    Overlay histogram showing HepG2 cells stained with ab110267 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110267, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

文献 (8)

发表研究结果有使用 ab110267？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab110267 被引用在 8 文献中.

  • Pajuelo Reguera D  et al. Cytochrome c Oxidase Subunit 4 Isoform Exchange Results in Modulation of Oxygen Affinity. Cells 9:N/A (2020). PubMed: 32075102
  • Chang FW  et al. Estrogen Enhances the Expression of the Multidrug Transporter Gene ABCG2-Increasing Drug Resistance of Breast Cancer Cells through Estrogen Receptors. Int J Mol Sci 18:N/A (2017). PubMed: 28098816
  • Chen X  et al. Artesunate Activates the Intrinsic Apoptosis of HCT116 Cells through the Suppression of Fatty Acid Synthesis and the NF-?B Pathway. Molecules 22:N/A (2017). PubMed: 28786914
  • Pérez-Pérez R  et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . PubMed: 27545886
  • Sohal RS  et al. Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster. Mech Ageing Dev 129:558-61 (2008). PubMed: 18538373
  • Janssen GM  et al. The A3243G tRNALeu(UUR) mutation induces mitochondrial dysfunction and variable disease expression without dominant negative acting translational defects in complex IV subunits at UUR codons. Hum Mol Genet 16:2472-81 (2007). PubMed: 17656376
  • Amaral A  et al. The expression of polymerase gamma and mitochondrial transcription factor A and the regulation of mitochondrial DNA content in mature human sperm. Hum Reprod 22:1585-96 (2007). PubMed: 17339235
  • Lock RB & Murphy KM Immunodetecting members of the Bcl-2 family of proteins. Methods Mol Med 111:83-96 (2005). PubMed: 15911974

客户评价及客户问答

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Question

We are wondering if the following antibodies can be purchased in a PBS buffer only format:



ab14734 anti-VDAC1 clone 20B12AF2



If possible, please provided pricing and lead times.



Please let me know if there may be any questions.



ab110267 anti-cytochrome C oxidase clone 3G5F7G3




Thanks very much for your help.

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Answer

Thank you for contacting Abcam.

We can look into custom formulation batches if you were interested in ordering 1mg or more of the antibody, but for smaller quantities we do not offer this service.

If however you only need 1 or 2vials of the antibody, then I would recommend you use one of our purification kits that will allow you to do a buffer exchange, so that you can get the antibody in PBS:

https://www.abcam.com/Mouse-Antibody-Purification-Kit-ab128745.html



If there is anything else I can help you with, or if you are interested in a bulk custom order, then please let me know.

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