Anti-Cytochrome C抗体(ab18738)
Key features and details
- Rabbit polyclonal to Cytochrome C
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cytochrome C抗体
参阅全部 Cytochrome C 一抗 -
描述
兔多克隆抗体to Cytochrome C -
宿主
Rabbit -
特异性
This antibody detects the 15 kDa cytochrome C. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Chicken
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免疫原
Synthetic peptide corresponding to Rat Cytochrome C. Corresponding to residues surrounding amino acid 70 of rat Cytochrome C.
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阳性对照
- WB: Jurkat cell lysate and 3T3 cell lysate. Rat kidney cell lysate.
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 3025 Cytochrome c Antibody. 3025-100 is the same size as the 100 µg size of ab18738.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18738于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 12 kDa.
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| 说明 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 12 kDa. |
靶标
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功能
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. -
疾病相关
Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation. -
序列相似性
Belongs to the cytochrome c family. -
翻译后修饰
Binds 1 heme group per subunit. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 420624 Chicken
- Entrez Gene: 54205 Human
- Entrez Gene: 25309 Rat
- Omim: 123970 Human
- SwissProt: P99999 Human
- SwissProt: P62898 Rat
- Unigene: 437060 Human
- Unigene: 617193 Human
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别名
- CYC antibody
- CYC_HUMAN antibody
- CYCS antibody
see all
图片
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Western blot - Anti-Cytochrome C antibody (ab18738)
Western Blot analysis of Left Lane: Jurkat cell lysate and Right Lane: 3T3 cell lysates labeling Cytochrome C with ab18738.
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Western blot - Anti-Cytochrome C antibody (ab18738)Anti-Cytochrome C antibody (ab18738) + Rat kidney cell lysates
Predicted band size: 12 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab18738 被引用在 11 文献中.
- E L et al. MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1. Exp Ther Med 17:2152-2160 (2019). PubMed: 30783480
- Liu H et al. The ginsenoside Rk3 exerts anti-esophageal cancer activity in vitro and in vivo by mediating apoptosis and autophagy through regulation of the PI3K/Akt/mTOR pathway. PLoS One 14:e0216759 (2019). PubMed: 31091245
- Kalpage HA et al. Serine-47 phosphorylation of cytochrome c in the mammalian brain regulates cytochrome c oxidase and caspase-3 activity. FASEB J 33:13503-13514 (2019). PubMed: 31570002
- Stancic A et al. A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection. Comp Biochem Physiol B Biochem Mol Biol 219-220:1-9 (2018). WB . PubMed: 29501789
- Dotiwala F et al. Granzyme B Disrupts Central Metabolism and Protein Synthesis in Bacteria to Promote an Immune Cell Death Program. Cell 171:1125-1137.e11 (2017). PubMed: 29107333