重组Anti-Cystatin C抗体[EPR4502] (ab133495)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4502] to Cystatin C
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cystatin C抗体[EPR4502]
参阅全部 Cystatin C 一抗 -
描述
兔单克隆抗体[EPR4502] to Cystatin C -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human Cystatin C aa 1-150.
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阳性对照
- Human testis, Human brain, PC3 and HepG2 lysates; Human ovarian tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4502 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133495于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. -
组织特异性
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. -
疾病相关
Amyloidosis 6
Macular degeneration, age-related, 11 -
序列相似性
Belongs to the cystatin family. -
翻译后修饰
The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 1471 Human
- Omim: 604312 Human
- SwissProt: P01034 Human
- Unigene: 304682 Human
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别名
- AD 8 antibody
- AD8 antibody
- Amyloid angiopathy and cerebral hemorrhage antibody
see all
图片
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All lanes : Anti-Cystatin C antibody [EPR4502] (ab133495) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Human brain lysate
Lane 3 : PC3 lysate
Lane 4 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4502] (ab133495)
Immunohistochemical analysis of Cystatin C in paraffin embedded Human ovarian tissue, using ab133495 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab133495 被引用在 3 文献中.
- Pires D et al. Development of Chitosan Particles Loaded with siRNA for Cystatin C to Control Intracellular Drug-Resistant Mycobacterium tuberculosis. Antibiotics (Basel) 12:N/A (2023). PubMed: 37107091
- Ahn SB et al. Potential early clinical stage colorectal cancer diagnosis using a proteomics blood test panel. Clin Proteomics 16:34 (2019). PubMed: 31467500
- Vartanian S et al. Application of Mass Spectrometry Profiling to Establish Brusatol as an Inhibitor of Global Protein Synthesis. Mol Cell Proteomics 15:1220-31 (2016). PubMed: 26711467