AB217569

重组Anti-Cystatin C抗体[EPR4413] - BSA and Azide free

Anti-Cystatin C antibody [EPR4413] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal Cystatin C antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)

Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109508 at adilution of 1/250.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109508).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)

Immunohistochemistry analysis of paraffin-embedded human kidney tissue sections labelling Cystatin C with ab109508 at 1/8000 dilution. The section was incubated with ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Positive staining on human kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. This data was developed using ab109508, the same antibody clone in a different buffer formulation.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)

Immunohistochemistry analysis of paraffin-embedded mouse kidney tissue sections labelling Cystatin C with ab109508 at 1/8000 dilution. The section was incubated with ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Positive staining on mouse kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. This data was developed using ab109508, the same antibody clone in a different buffer formulation.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)

Immunohistochemistry analysis of paraffin-embedded rat kidney tissue sections labelling Cystatin C with ab109508 at 1/8000 dilution. The section was incubated with ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Positive staining on rat kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. This data was developed using ab109508, the same antibody clone in a different buffer formulation.

Unknown

Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)

This data was developed using ab109508, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569) at 1/10000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Predicted band size: 15 kDa

Observed band size: 13 kDa

false

Lab

Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)

This data was developed using ab109508, the same antibody clone in a different buffer formulation.

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : CST3 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : Caco2 whole cell lysate (20 μg)
Lane 4 : U20S whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab109508 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109508 was shown to specifically react with CST3 when CST3 knockout samples were used. Wild-type and CST3 knockout samples were subjected to SDS-PAGE. ab109508 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)

Predicted band size: 15 kDa

false

Lab

Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST This data was developed using ab109508, the same antibody clone in a different buffer formulation.

Lane 1:

Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569) at 1/10000 dilution

Lane 1:

Western blot - Anti-Cystatin C antibody [EPR4413] (<a href='/products/primary-antibodies/cystatin-c-antibody-epr4413-ab109508'>ab109508</a>) at 1/10000 dilution

All lanes:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 15 kDa

Observed band size: 13 kDa

false

Exposure time: 80s

Lab

Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST This data was developed using ab109508, the same antibody clone in a different buffer formulation.

Lane 1:

Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569) at 1/10000 dilution

Lane 1:

Western blot - Anti-Cystatin C antibody [EPR4413] (<a href='/products/primary-antibodies/cystatin-c-antibody-epr4413-ab109508'>ab109508</a>) at 1/10000 dilution

All lanes:

Mouse thymus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 15 kDa

Observed band size: 13 kDa

false

Exposure time: 3min

不同偶联物与剂型 (1)

Unconjugated

Anti-Cystatin C antibody [EPR4413]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4413

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab217569 is the carrier-free version of ab109508.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

See full target information CST3

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