Anti-Cystatin-B抗体[2F1] (ab72353)
![Immunocytochemistry/ Immunofluorescence - Anti-Cystatin-B antibody [2F1] (ab72353)](https://www.abcam.cn/ps/products/72/ab72353/Images/ab72353-353837-AntiCystatinB-antibody-2F1-immunocytochemistry.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cystatin-B antibody [2F1] (ab72353)")
Key features and details
- Mouse monoclonal [2F1] to Cystatin-B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-Cystatin-B抗体[2F1]
参阅全部 Cystatin-B 一抗 -
描述
小鼠单克隆抗体[2F1] to Cystatin-B -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Cystatin-B.
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阳性对照
- A549 cell lysate
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常规说明
This product was changed from ascites to tissue culture supernatant on 08/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
2F1 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72353于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
|
| ICC/IF |
1/100.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa). |
|
ICC/IF
1/100. |
靶标
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功能
This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. -
疾病相关
Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) [MIM:254800]. EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop. -
序列相似性
Belongs to the cystatin family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1476 Human
- Omim: 601145 Human
- SwissProt: P04080 Human
- Unigene: 695 Human
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别名
- CHROW21 antibody
- CPI B antibody
- CPI-B antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Cystatin-B antibody [2F1] (ab72353)
ICC/IF analysis of CSTB in A549 cells. The cell was stained with ab72353(1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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![Western blot - Anti-Cystatin-B antibody [2F1] (ab72353)](https://www.abcam.cn/ps/products/72/ab72353/Images/ab72353-353836-AntiCystatinB-antibody-2F1-westernblot.jpg)
Western blot - Anti-Cystatin-B antibody [2F1] (ab72353)All lanes : Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : U87MG cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 11 kDa -
![Western blot - Anti-Cystatin-B antibody [2F1] (ab72353)](https://www.abcam.cn/ps/products/72/ab72353/Images/Wb cystatin.jpg)
Western blot - Anti-Cystatin-B antibody [2F1] (ab72353)Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution + A549 cells lysate at 35 µg
Secondary
goat anti-mouse secondary antibody conjugated to HRP
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab72353 被引用在 1 文献中.
- Burgener A et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol 87:5141-50 (2013). Human . PubMed: 23449785