Anti-Cystathionase/CTH抗体[2E12-1C10] (ab54573)

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We do have full length human Cystathionase in our catalog as ab114582.

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I did search for the lot number that you provided and found that a product with this lot number was purchased on 22 January 2011 for UNM. While no longer under our Abpromise, this product should remain stable for up to 5 years if upon delivery it was aliquoted and stored at -20°C or -80°C, taking care to avoid repeated freeze / thaw cycles.

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Thank you very much for keeping me up to date with the performance of the antibodies. I am very happy to hear you could obtain satisfactory results from ab54573. On the other hand it is regrettable ab80643 didn’t perform accordingly. I appreciate the time you have spent in the laboratory and understand your concerns. I can suggest you have regrettably received a bad vial of ab80643. As the order for this antibody was placed within the last 6 months, it is still covered by our Abpromise guarantee, and if you wish to, I can send you a free replacement for this antibody (from a different lot) a reimbursement or a Credit Note in compensation. I am very sorry for the inconveniences, and I will be happy to assist you with this problem, and give a quick and efficient solution. Therefore I look forward to hearing from you letting me know how you would like to proceed. Have a nice day.

Thanks for your reply. We don’t have any evidence of the expression of cystathionase in HUVECs. If you have data to support the presence of the protein in the cell line, but not in high quantities, it would be better loading bigger amounts of sample per lane. Attending to the antibodies datasheets, for ab54573, K-562 cell lysate was used to detect Cystathionase. We do have in our catalogue K562 cell lysates that could be used as positive controls. The link to the search result is: https://www.abcam.com/index.html?pageconfig=searchresults&search=K 562&pt=12&sk=targ&sv=K562&sn=K562&l=1&fViewMore=1 For ab80643 the suggested positive control is Transfected 293T cell lysate. Unfortunately, we have 293T cell lysate in out catalogue, but not the transfected one, that would be the one recommended to work with. HeLa lysate can also be used as a positive control. There are some HeLa lysates available in the catalogue, if you wish to have a look: https://www.abcam.com/index.html?pageconfig=searchresults&search=HeLa&pt=12&sk=app&sv=69&sn=WB&l=2&fViewMore=1 I would encourage you to perform the WB again following the recommendations given (higher protein amount, using phosphatase inhibitors, concentrating the ab dilution as well as increasing the secondary concentration) and maybe using any of these lysates to check whether the problem comes from the antibody or from the protein expression level. In case you can’t notice any improvement, please contact me again and I’ll try to give a solution as soon as possible.

Thank you for sending these additional details. If we assume the cystathionase in your samples is intact and not degraded then the antibody is probably the issue. I think your protocol is correct, and the Hela lysate, at least, should be giving a signal. Would you be interested in trying the rabbit polyclonal, ab80643, as a free-of-charge replacement? The only data we have is a blot against transfected 293 cells, but we will offer our guarantee (replacement or credit or refund within 6 months of purchase, after considering the protocol) for reactivity with endogenous cystathionase in human samples, e.g. HeLa. Reactivity with other species is predicted based on homology with the immunogen but has not been tested.