Anti-CYP3A5抗体[F18 P3 B6] (ab56076)
![Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (ab56076)](https://www.abcam.cn/ps/products/56/ab56076/Images/ab56076-3.jpg "Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (ab56076)")
Key features and details
- Mouse monoclonal [F18 P3 B6] to CYP3A5
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-CYP3A5抗体[F18 P3 B6]
参阅全部 CYP3A5 一抗 -
描述
小鼠单克隆抗体[F18 P3 B6] to CYP3A5 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human CYP3A5 (C terminal).
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阳性对照
- Oesophageal Squamous cell carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
克隆
单克隆 -
克隆编号
F18 P3 B6 -
骨髓瘤
Ag8.653 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab56076于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
|
|
| IHC-P |
Use a concentration of 1 - 5 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
|
IHC-P
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1577 Human
- Omim: 605325 Human
- SwissProt: P20815 Human
- Unigene: 695915 Human
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别名
- Aryl hydrocarbon hydroxylase antibody
- CP35 antibody
- CP3A5_HUMAN antibody
see all
图片
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Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (ab56076)All lanes : Anti-CYP3A5 antibody [F18 P3 B6] (ab56076) at 2 µg/ml
Lane 1 : CYP3A4 recombinant
CYP450 protein
Lane 2 : CYP3A5 recombinant
CYP450 protein
Lane 3 : CYP3A7 recombinant
CYP450 protein
Predicted band size: 57 kDa
Observed band size: 57 kDa -
![Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (ab56076)](https://www.abcam.cn/ps/products/56/ab56076/Images/ab56076-4.jpg)
Western blot - Anti-CYP3A5 antibody [F18 P3 B6] (ab56076)Lane 1 : Molecular weight markers
Lanes 2-10 : Anti-CYP3A5 antibody [F18 P3 B6] (ab56076) at 2 µg/ml
Lane 2 : CYP1A1 recombinant protein
Lane 3 : CYP2C18 recombinant protein
Lane 4 : CYP2C19 recombinant protein
Lane 5 : CYP2D6 recombinant protein
Lane 6 : CYP4F2 recombinant protein
Lane 7 : CYP4F3 recombinant protein
Lane 8 : CYP3A5 recombinant protein
Lane 9 : CYP3A7 recombinant protein
Lane 10 : Negative control
Predicted band size: 57 kDa
Observed band size: 57 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [F18 P3 B6] (ab56076)](https://www.abcam.cn/ps/products/56/ab56076/Images/ab56076ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [F18 P3 B6] (ab56076)Oesophageal squamous cell carcinoma tissue stained with ab56076 at 5 µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab56076 被引用在 1 文献中.
- Yan F et al. A dual functional probe for assessing human CYP450 3A5 and 3A enzymes bioactivities. Future Med Chem 11:2891-2903 (2019). PubMed: 31702381