重组CYP3A5抗体[EPR4396](ab108624)|Abcam中文官网

Product price, shipping and contact information

Currently unavailable

抱歉，我们目前无法显示此内容。

请联系我们以完成下单，或者之后再尝试访问。

正在加载规格和价格。。。

Abpromise

产品质量保证，客户专家支持，

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4396] to CYP3A5
Suitable for: WB, IP, IHC-P
Reacts with: Human

Carrier Free

产品名称

Anti-CYP3A5抗体[EPR4396]
参阅全部 CYP3A5 一抗
描述

兔单克隆抗体[EPR4396] to CYP3A5
宿主

Rabbit
经测试应用

适用于: WB, IP, IHC-Pmore details
种属反应性

与反应: Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Human fetal liver and colon tissue lysates. IHC-P: Human liver and kidney tissues. IP: Human fetal liver tissue lysate.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR4396
同种型

IgG
研究领域

Alternative Versions
- Anti-CYP3A5 antibody [EPR4396] - BSA and Azide free (ab246337)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise™承诺保证使用ab108624于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/1000. Predicted molecular weight: 57 kDa. For unpurified use at 1/400.
IP		1/50. For unpurified use at 1/20.
IHC-P		1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/80.

说明
WB 1/1000. Predicted molecular weight: 57 kDa. For unpurified use at 1/400.
IP 1/50. For unpurified use at 1/20.
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/80.

功能

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
序列相似性

Belongs to the cytochrome P450 family.
细胞定位

Endoplasmic reticulum membrane. Microsome membrane.
Target information above from: UniProt accession P20815 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 1577 Human
- Omim: 605325 Human
- SwissProt: P20815 Human
- Unigene: 695915 Human
别名
- Aryl hydrocarbon hydroxylase antibody
- CP35 antibody
- CP3A5_HUMAN antibody
- CYP3A5 antibody
- CYPIIIA5 antibody
- Cytochrome P450 3A5 antibody
- Cytochrome P450 antibody
- Cytochrome P450 family 3 subfamily A polypeptide 5 antibody
- Cytochrome P450 HLp2 antibody
- Cytochrome P450 subfamily IIIA (niphedipine oxidase) polypeptide 5 antibody
- Cytochrome P450-PCN3 antibody
- DKFZp686L16231 antibody
- Flavoprotein linked monooxygenase antibody
- FLJ31317 antibody
- HLp2 antibody
- Microsomal monooxygenase antibody
- Niphedipine oxidase antibody
- P450PCN3 antibody
- PCN3 antibody
- Xenobiotic monooxygenase antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CYP3A5 with unpurified ab108624. Positive staining is shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)

Anti-CYP3A5 antibody [EPR4396] (ab108624) at 1/400 dilution (unpurified) + Human fetal liver tissue lysate at 20 µg

Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 57 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624)

ab108624 (unpurified) at 1/20 immunoprecipitating CYP3A5 in human fetal liver tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CYP3A5 with unpurified ab108624 at 1/80. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)

Anti-CYP3A5 antibody [EPR4396] (ab108624) at 1/1000 dilution (purified) + Human fetal liver tissue lysate at 20 µg

Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 57 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunoprecipitation - Anti-CYP3A5 antibody [EPR4396] (ab108624)

ab108624 (purified) at 1/50 immunoprecipitating CYP3A5 in human fetal liver tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human kidney tissue labelling CYP3A5 with unpurified ab108624. Positive staining is shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CYP3A5 antibody [EPR4396] (ab108624)

All lanes : Anti-CYP3A5 antibody [EPR4396] (ab108624) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal colon tissue lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human breast tissue labelling CYP3A5 with unpurified ab108624. Negative staining is shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CYP3A5 with unpurified ab108624. Negative staining is shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labelling CYP3A5 with unpurified ab108624. Negative staining is shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP3A5 antibody [EPR4396] (ab108624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CYP3A5 with purified ab108624 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Anti-CYP3A5 antibody [EPR4396] (ab108624)

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab108624？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab108624 被引用在 8 文献中.

Zhou X et al. Persister cell phenotypes contribute to poor patient outcomes after neoadjuvant chemotherapy in PDAC. Nat Cancer 4:1362-1381 (2023). PubMed: 37679568
Wright WC et al. Clobetasol Propionate Is a Heme-Mediated Selective Inhibitor of Human Cytochrome P450 3A5. J Med Chem 63:1415-1433 (2020). PubMed: 31965799
Wang L et al. miR-543 acts as a novel oncogene in oral squamous cell carcinoma by targeting CYP3A5. Oncol Rep 42:973-990 (2019). PubMed: 31322243
Ou B et al. TLR9 rs352139 Genetic Variant Promotes Tacrolimus Elimination in Chinese Liver Transplant Patients During the Early Posttransplantation Period. Pharmacotherapy 39:67-76 (2019). PubMed: 30537010
Zhang T et al. Association of donor small ubiquitin-like modifier 4 rs237025 genetic variant with tacrolimus elimination in the early period after liver transplantation. Liver Int 38:724-732 (2018). PubMed: 28941036
Oladimeji PO et al. RNA interference screen identifies NAA10 as a regulator of PXR transcription. Biochem Pharmacol 160:92-109 (2018). PubMed: 30566892
Zhang X et al. A tryptophan derivative, ITE, enhances liver cell metabolic functions in vitro. Int J Mol Med 39:101-112 (2017). PubMed: 27959388
Fanni D et al. Immunohistochemical markers of CYP3A4 and CYP3A7: a new tool towards personalized pharmacotherapy of hepatocellular carcinoma. Eur J Histochem 60:2614 (2016). PubMed: 27349315

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

Question

Dear Sir or Madam,

Do you know if it could be possible to use the MAb-CYP3A5 to perform inhibitions of the CYP3A5 enzymatic activity in human liver microsomes? If so, do you have a recommended protocol?

Thank you very much in advance for your attention.

Best Regards,

Abcam community

Verified customer

Asked on Jul 18 2012

Answer

Thank you for your inquiry.
I am sorry to confirm that we have no reports of the suitability of ab108624 (Anti-Cytochrome P450 3A5 antibody [EPR4396]) for inhibitory assays.
We do not know if it would work and I have to admit that I am unable to make a prediction. The immunogen of this antibody is in the region of amino acid 240 to 280. I could not find any evidence that this area is responsible for the enzymatic activity.
I am sorry I did not have a positive answer for you on this occasion a and hope this information is nevertheless helpful.

Abcam Scientific Support

回复于 Jul 18 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

应用

The Abpromise guarantee

靶标

功能

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (8)

客户评价及客户问答