CYP2D6重组抗体[EPR17868]_CYP2D6抗体(ab185625)| Abcam中文官网

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Immunocytochemistry/ Immunofluorescence - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17868] to CYP2D6
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
Reacts with: Human

Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free

产品名称

Anti-CYP2D6抗体[EPR17868]
参阅全部 CYP2D6 一抗
描述

兔单克隆抗体[EPR17868] to CYP2D6
宿主

Rabbit
经测试应用

适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
种属反应性

与反应: Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: CYP2D6 transfected 293T lysate; Human fetal liver lysate; HeLa, K562, HepG2 and HT-29 whole cell lysates. IHC-P: Human liver, kidney and hepatocellular carcinoma tissues. ICC/IF: HT-29 cells. Flow Cyt (intra): HT-29 cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR17868
同种型

IgG
研究领域
- Neuroscience
- Processes

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise™承诺保证使用ab185625于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/1000. Detects a band of approximately 55, 50 kDa (predicted molecular weight: 55, 50 kDa).
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/1000.
Flow Cyt (Intra)		1/300. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

说明
WB 1/1000. Detects a band of approximately 55, 50 kDa (predicted molecular weight: 55, 50 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/1000.
Flow Cyt (Intra) 1/300. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

功能

Responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It is involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants.
序列相似性

Belongs to the cytochrome P450 family.
细胞定位

Endoplasmic reticulum membrane. Microsome membrane.
Target information above from: UniProt accession P10635 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 1565 Human
- Omim: 124030 Human
- SwissProt: P10635 Human
- Unigene: 648256 Human
别名
- Cholesterol 25-hydroxylase antibody
- CP2D6_HUMAN antibody
- CPD6 antibody
- CYP2D antibody
- CYP2D6 antibody
- CYP2D7AP antibody
- CYP2D7BP antibody
- CYP2D7P2 antibody
- CYP2D8P2 antibody
- CYP2DL1 antibody
- CYPIID6 antibody
- Cytochrome P450 2D6 antibody
- Cytochrome P450 DB1 antibody
- Cytochrome P450 family 2 subfamily D member 6 antibody
- Cytochrome P450 family 2 subfamily D polypeptide 6 antibody
- Cytochrome P450-DB1 antibody
- Debrisoquine 4 hydroxylase antibody
- Debrisoquine 4-hydroxylase antibody
- Flavoprotein linked monooxygenase antibody
- Microsomal monooxygenase antibody
- P450 DB1 antibody
- P450C2D antibody
- P450DB1 antibody
- Xenobiotic monooxygenase antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cells) cells labeling CYP2D6 with ab185625 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic staining on HT-29 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab185625 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CYP2D6 with ab185625 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human liver is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CYP2D6 with ab185625 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human kidney is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling CYP2D6 with ab185625 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HT-29 (Human colorectal adenocarcinoma cells) cells labeling CYP2D6 with ab185625 at 1/300 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Western blot - Anti-CYP2D6 antibody [EPR17868] (ab185625)

All lanes : Anti-CYP2D6 antibody [EPR17868] (ab185625) at 1/10000 dilution

Lane 1 : Full-length CYP2D6 transfected 293T cell lysate
Lane 2 : Non-transfected 293T lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55, 50 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Full-length CYP2D6 293T overexpressed lysates contain GFP tagged-aa1-497.
Western blot - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Anti-CYP2D6 antibody [EPR17868] (ab185625) at 1/10000 dilution + Human fetal liver tissue lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55, 50 kDa
Observed band size: 50,55 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Based on the sequence analysis, ab185625 recognizes two isoforms with the predicted MWs of 55kDa and 50kDa respectively.
Western blot - Anti-CYP2D6 antibody [EPR17868] (ab185625)

All lanes : Anti-CYP2D6 antibody [EPR17868] (ab185625) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55, 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CYP2D6 antibody [EPR17868] (ab185625)

Anti-CYP2D6 antibody [EPR17868] (ab185625) at 1/1000 dilution + HT-29 (Human colorectal adenocarcinoma cells) whole cell lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55, 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CYP2D6 antibody [EPR17868] (ab185625)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab185625？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab185625 被引用在 3 文献中.

Zhou L et al. A Metabolism-Related Gene Landscape Predicts Prostate Cancer Recurrence and Treatment Response. Front Immunol 13:837991 (2022). PubMed: 35359973
Chattopadhyay M et al. Mitochondrially targeted cytochrome P450 2D6 is involved in monomethylamine-induced neuronal damage in mouse models. J Biol Chem 294:10336-10348 (2019). PubMed: 31113867
Ueno M et al. Alendronate inhalation ameliorates elastase-induced pulmonary emphysema in mice by induction of apoptosis of alveolar macrophages. Nat Commun 6:6332 (2015). PubMed: 25757189

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

应用

The Abpromise guarantee

靶标

功能

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (3)

客户评价及客户问答