重组Anti-CYP2C19抗体[EPR6576]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] (AB137015)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling CYP2C19 with unpurified ab137015 at 1/250 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] (AB137015)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CYP2C19 with Purified ab137015 at 1 : 1000 dilution (0.49 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] (AB137015)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling CYP2C19 with Purified ab137015 at 1 : 1000 dilution (0.49 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C19 antibody [EPR6576] (AB137015)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling CYP2C19 with Purified ab137015 at 1 : 1000 dilution (0.49 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.

• WB

Unknown

Western blot - Anti-CYP2C19 antibody [EPR6576] (AB137015)

Blocking and diluting buffer : 5% NFDM/TBST.
The doublet should be due to the cross-reaction with family members.

All lanes:

Western blot - Anti-CYP2C19 antibody [EPR6576] (ab137015) at 0.5 µg/mL

Lane 1:

Human fetal liver lysates at 15 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates, negative control at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

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• WB

Unknown

Western blot - Anti-CYP2C19 antibody [EPR6576] (AB137015)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CYP2C19 antibody [EPR6576] (ab137015) at 0.5 µg/mL

Lane 1:

Mouse liver lysates at 15 µg

Lane 2:

Rat liver lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

false

• WB

Unknown

Western blot - Anti-CYP2C19 antibody [EPR6576] (AB137015)

All lanes:

Western blot - Anti-CYP2C19 antibody [EPR6576] (ab137015) at 1/1000 dilution

Lane 1:

Mouse liver lysate at 10 µg

Lane 2:

Rat liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 56 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CYP2C19 antibody [EPR6576] (AB137015)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

• WB

CiteAb

Western blot - Anti-CYP2C19 antibody [EPR6576] (AB137015)

CYP2C19 western blot using anti-CYP2C19 antibody [EPR6576] ab137015. Publication image and figure legend from Grant, M. K. O., Abdelgawad, I. Y., et al., 2020, Int J Mol Sci, PubMed 32074957.

ab137015 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137015 please see the product overview.

Effect of DOX treatment on the expression of the Cyp2c sub-family in the liver. Livers were collected from male or female C57Bl/6 mice 24 or 72 h following the administration of a single intraperitoneal injection of 20 mg/kg DOX or saline, and total proteins and RNA were isolated. (A) Gene expression of Cyp2c29 was determined by real-time PCR (n = 8 per group) and (B) protein levels of total Cyp2c were determined by western blot (n = 4 per group) in samples collected 24 h following DOX treatment. (C) Gene expression of Cyp2c29 (n = 4–5 per group) and (D) protein levels of Cyp2c (n = 4–5 per group) were determined in samples collected 72 h following DOX treatment. PCR results were normalized to beta-actin, and protein expression was normalized to alpha-tubulin. Results of all groups are expressed relative to the male control. Data are presented as the mean ± SEM. * p < 0.05, compared to control mice of the same sex; # p < 0.05, compared to male DOX-treated mice.

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