Anti-CYP2C11抗体(ab3571)
Key features and details
- Rabbit polyclonal to CYP2C11
- Suitable for: IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-CYP2C11抗体 -
描述
兔多克隆抗体to CYP2C11 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
This product was produced with the following immunogens:
Synthetic peptide corresponding to Rat CYP2C11 aa 1-100.
Synthetic peptide corresponding to Rat CYP2C11 aa 450-550. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Whole antiserum -
Primary antibody说明
The Cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA’s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). EET’s, which exhibit vasodilation activity, are formed when an epoxide group is inserted between the unsaturated carbons of AA in positions 5,6; 8,9; 11,12; 14,15. EET’s are produced in cerebral cortical tissue, coronary arteries and vascular endothelium. EET’s are converted from AA by the 2C11 family of P450’s whose expression is induced by testosterone and is therefore not generally found in females. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/20 - 1/200.
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说明 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/20 - 1/200. |
靶标
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功能
Metabolizes testosterone mainly in positions 2 alpha and 16 alpha. -
组织特异性
Liver; male-specific. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 29277 Rat
- SwissProt: P08683 Rat
- Unigene: 10870 Rat
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别名
- CP2CB_RAT antibody
- Cyp2c antibody
- Cyp2c11 antibody
see all
图片
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ab3571 staining CYP2C11 (green) in HeLa (Human epithelial adenocarcinoma cell line) cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)
ab3571 staining CYP2C11 in the cytoplasm of rat liver tissue (right) compared with a negative control in the absence of primary antibody (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were then blocked in 3% H2O2-methanol for 15 min at room temperature. Sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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ab3571 staining CYP2C11 (green) in PC-12 (Rat adrenal gland pheochromocytoma cell line) cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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ab3571 staining CYP2C11 (green) in H-4-II-E (Rat hepatoma cell line) cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)
ab3571 staining CYP2C11 in the cytoplasm of rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were then blocked in 3% H2O2-methanol for 15 min at room temperature. Sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)IHC image of ab3571 staining in human renal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3571, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab3571 被引用在 13 文献中.
- Li Z et al. Disease Status-Dependent Drug-Herb Interactions: NASH Lowered the Risk of Hepatotoxicity in Rats Coadministered With Simvastatin and Gardenia jasminoides J. Ellis. Front Pharmacol 12:622040 (2021). PubMed: 33967756
- Kojina M et al. Acute Peripheral Inflammation Increases Plasma Concentration of Hypoglycemic Agent Nateglinide with Decreased Hepatic Drug-Metabolizing Activity in Rats. Biol Pharm Bull 44:96-102 (2021). PubMed: 33390555
- Doan TNK et al. Differential Effects of 1a,25-Dihydroxyvitamin D3 on the Expressions and Functions of Hepatic CYP and UGT Enzymes and Its Pharmacokinetic Consequences In Vivo. Pharmaceutics 12:N/A (2020). PubMed: 33238436
- Li H et al. Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction through AA Metabolism Modulated CYPs Pathway. Int J Biol Sci 15:2037-2050 (2019). PubMed: 31592228
- Xiao M et al. Impact of the Chinese herbal medicines on dual antiplatelet therapy with clopidogrel and aspirin: Pharmacokinetics and pharmacodynamics outcomes and related mechanisms in rats. J Ethnopharmacol 235:100-110 (2019). PubMed: 30710735