Anti-CYP1A1抗体(ab126887)
Key features and details
- Goat polyclonal to CYP1A1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-CYP1A1抗体
参阅全部 CYP1A1 一抗 -
描述
山羊多克隆抗体to CYP1A1 -
宿主
Goat -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Mouse CYP1A1 aa 294-308 (internal sequence) (Cysteine residue).
Sequence:C-QDRKLDENANVQLSD
Database link: NP_001129531.1 -
阳性对照
- WB: Human and rat lung lysate. IHC-P: Human tonsil tissue. ICC/IF: NIH3T3 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
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| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 55 kDa).
1 hour primary incubation is recommended for this product. |
|
| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 55 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. -
组织特异性
Lung, lymphocytes and placenta. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1543 Human
- Entrez Gene: 13076 Mouse
- Entrez Gene: 24296 Rat
- Omim: 108330 Human
- SwissProt: P04798 Human
- SwissProt: P00184 Mouse
- SwissProt: P00185 Rat
- Unigene: 72912 Human
see all -
别名
- AHH antibody
- AHRR antibody
- Aryl hydrocarbon hydroxylase antibody
see all
图片
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Western blot - Anti-CYP1A1 antibody (ab126887)Anti-CYP1A1 antibody (ab126887) at 0.1 µg/ml + Rat lung lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1A1 antibody (ab126887)Immunohistochemistry analysis of paraffin embedded human tonsil tissue labeling CYP1A1 with ab126887. Heat induced antigen retrieval was performed with Tris-EDTA pH 9, HRP-Staining (1/500) (left).Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody (right). -
Immunocytochemistry/ Immunofluorescence - Anti-CYP1A1 antibody (ab126887)Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL). -
Western blot - Anti-CYP1A1 antibody (ab126887)Anti-CYP1A1 antibody (ab126887) at 0.1 µg/ml + Human Lung lysate (35 µg protein in RIPA buffer)
Predicted band size: 55 kDaDetected by chemiluminescence.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab126887 被引用在 2 文献中.
- Bouvier ML et al. Sex-dependent effects of long-term clozapine or haloperidol medication on red blood cells and liver iron metabolism in Sprague Dawley rats as a model of metabolic syndrome. BMC Pharmacol Toxicol 23:8 (2022). PubMed: 35033194
- Xie J et al. Taxifolin suppresses the malignant progression of gastric cancer by regulating the AhR/CYP1A1 signaling pathway. Int J Mol Med 48:N/A (2021). PubMed: 34490474