AB156016

重组Anti-CYFIP1抗体[EPR10782]

Anti-CYFIP1 antibody [EPR10782]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal CYFIP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

查看别名

KIAA0068, CYFIP1, Cytoplasmic FMR1-interacting protein 1, Specifically Rac1-associated protein 1, p140sra-1, Sra-1

4 Images

Flow Cytometry (Intracellular) - Anti-CYFIP1 antibody [EPR10782] (AB156016)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CYFIP1 antibody [EPR10782] (AB156016)

Intracellular Flow Cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling CYFIP1 with Purified ab156016 at 1/30 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-CYFIP1 antibody [EPR10782] (AB156016)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CYFIP1 antibody [EPR10782] (AB156016)

Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling CYFIP1 with Purified ab156016 at 1/50 dilution (5.56 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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Western blot - Anti-CYFIP1 antibody [EPR10782] (AB156016)

Blocking/Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CYFIP1 antibody [EPR10782] (ab156016) at 1/1000 dilution

Lane 1:

Human brain lysates at 20 µg

Lane 2:

Human lung lysates at 20 µg

Lane 3:

Mouse brain lysates at 20 µg

Lane 4:

Mouse lung lysates at 20 µg

Lane 5:

Mouse breast lysates at 20 µg

Lane 6:

Rat brain lysates at 20 µg

Lane 7:

Rat lung lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 145 kDa

Observed band size: 145 kDa

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Western blot - Anti-CYFIP1 antibody [EPR10782] (AB156016)

Blocking/Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CYFIP1 antibody [EPR10782] (ab156016) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 3:

A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 6:

C2C12 (Mouse myoblasts myoblast) whole cell lysates at 20 µg

Lane 7:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

Lane 9:

Rat-1 (Rat embryonic fibroblast) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 145 kDa

Observed band size: 145 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10782

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "For unpurified use at 1/250 - 1/500.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "For unpurified use at 1/10 - 1/100. <a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CYFIP1 also known as cytoplasmic FMR1-interacting protein 1 plays an important role in cellular processes including actin dynamics and translation regulation. This protein which has a mass of approximately 140 kDa interacts with FMRP and links to the ribosome controlling mRNA translation. CYFIP1 is expressed mostly in the brain but is also present in other tissues like blood. Researchers study CYFIP1 to understand its role in neural activity and cellular signaling processes.

Biological function summary

CYFIP1 operates as part of the WAVE regulatory complex which is critical for actin polymerization. This complex facilitates cytoskeletal reorganization essential for cellular movement and morphology. CYFIP1 influences synaptic plasticity an important process in neural signaling and cognitive functions. By interacting with other molecular components CYFIP1 impacts processes like memory formation and learning.

Pathways

CYFIP1 is integral to the Rac1 and Arp2/3 pathways which directly affect actin filament assembly. In these pathways CYFIP1 interacts with proteins such as NCKAP1 and ABI2 which play roles in the regulation of actin cytoskeleton reorganization. The involvement of CYFIP1 in these pathways highlights its importance in processes that require rapid cytoskeletal changes such as cell migration and synaptic growth.

CYFIP1's function draws a connection to neurodevelopmental disorders and schizophrenia. Alterations in the expression of CYFIP1 have been observed in these conditions suggesting a link between its dysregulation and abnormal brain development. Moreover its interaction with the FMRP protein ties it to Fragile X syndrome characterized by cognitive impairment. Research into these associations provides valuable insight into potential therapeutic targets for these complex conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Regulates formation of membrane ruffles and lamellipodia. Plays a role in axon outgrowth. Binds to F-actin but not to RNA. Part of the WAVE complex that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Regulator of epithelial morphogenesis. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity). May act as an invasion suppressor in cancers.

See full target information CYFIP1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:8187 PubMed38081847

2023

GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Zhu,Matthew E Combs,Juan Liu,Xue Bai,Wenbo B Wang,Laura E Herring,Jiandong Liu,Jason W Locasale,Dawn E Bowles,Ryan T Gross,Michelle Mendiola Pla,Christopher P Mack,Joan M Taylor

Biological psychiatry 95:676-686 PubMed37573007

2023

Loss of Function in the Neurodevelopmental Disease and Schizophrenia-Associated Gene CYFIP1 in Human Microglia-like Cells Supports a Functional Role in Synaptic Engulfment.

Applications

Unspecified application

Species

Unspecified reactive species

Steven D Sheridan,Joy E Horng,Hana Yeh,Liam McCrea,Jennifer Wang,Ting Fu,Roy H Perlis

Life sciences 270:119033 PubMed33497737

2021

Treadmill exercise enhances synaptic plasticity in the ischemic penumbra of MCAO mice by inducing the expression of Camk2a via CYFIP1 upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Weimin Shen,Lingqin Jin,Anqi Zhu,Yao Lin,Guoyuan Pan,Shanshan Zhou,Jingyan Cheng,Jieqiong Zhang,Fengxia Tu,Chan Liu,Qingfeng Xie,Xiang Chen

Cells 9: PubMed32646006

2020

The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie A Whitelaw,Karthic Swaminathan,Frieda Kage,Laura M Machesky

Translational psychiatry 9:29 PubMed30664619

2019

Behavioral training rescues motor deficits in Cyfip1 haploinsufficiency mouse model of autism spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Sven O Bachmann,Monika Sledziowska,Ellen Cross,Shireene Kalbassi,Sophie Waldron,Fangli Chen,Adam Ranson,Stéphane J Baudouin

View all publications

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重组Anti-CYFIP1抗体[EPR10782]