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重组RabMAb

重组Anti-Cyclophilin A抗体[EPR7511] (ab126738)

Submit a review Submit a question References (6)
Western blot - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
  • Western blot - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
  • OI-RD Scanning - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
  • Anti-Cyclophilin A antibody [EPR7511] (ab126738)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7511] to Cyclophilin A
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

概述

  • 产品名称

    Anti-Cyclophilin A抗体[EPR7511]
    参阅全部 Cyclophilin A 一抗

  • 描述

    兔单克隆抗体[EPR7511] to Cyclophilin A

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF or IP

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • K562, HeLa, Jurkat, and HepG2 whole cell lysate (ab7900). Human breast carcinoma tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126738于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.

靶标

图片

  • Western blot - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    Western blot - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    All lanes : Anti-Cyclophilin A antibody [EPR7511] (ab126738) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa


    Exposure time: 180 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Low expression control: mouse heart.
    In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)

    Immunohistochemistry analysis of paraffin-embedded human kidney tissue sections labeling Cyclophilin A with ab126738 at 1/1000 (0.214 μg/mL) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Hematoxylin was as the counterstain. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    Positive staining on the human kidney. The section was incubated with ab126738 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)

    Immunohistochemistry analysis of paraffin-embedded mouse kidney tissue sections labeling Cyclophilin A with ab126738 at 1/1000 (0.214 μg/mL) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Hematoxylin was as the counterstain. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    Positive staining on the mouse kidney. The section was incubated with ab126738 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Western blot - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    Western blot - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    All lanes : Anti-Cyclophilin A antibody [EPR7511] (ab126738) at 1/1000 dilution

    Lane 1 : K562 lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat lysate
    Lane 4 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)

    ab126738 at 1/100 dilution, staining Cyclophilin A in Formalin fixed Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)

    Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue sections labeling Cyclophilin A with ab126738 at 1/1000 (0.214 μg/mL) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Hematoxylin was as the counterstain. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    Low expression: weak staining on the mouse cardiac muscle. The section was incubated with ab126738 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin A antibody [EPR7511] (ab126738)

    Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue sections labeling Cyclophilin A with ab126738 at 1/1000 (0.214 μg/mL) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Hematoxylin was as the counterstain. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    Low expression: weak staining on the human skeletal muscle. The section was incubated with ab126738 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • OI-RD Scanning - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    OI-RD Scanning - Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Cyclophilin A antibody [EPR7511] (ab126738)
    Anti-Cyclophilin A antibody [EPR7511] (ab126738)

文献 (6)

发表研究结果有使用 ab126738？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab126738 被引用在 6 文献中.

  • Kwon H  et al. Peptidyl-prolyl cis/trans isomerase Pin1 interacts with hepatitis B virus core particle, but not with HBc protein, to promote HBV replication. Front Cell Infect Microbiol 13:1195063 (2023). PubMed: 37404723
  • Shaaya ES  et al. Placental Cyclophilin A Expression in Pregnancies Complicated with Hypertension. Int J Environ Res Public Health 19:N/A (2022). PubMed: 35564847
  • Du L & Gao Y PGM5-AS1 impairs miR-587-mediated GDF10 inhibition and abrogates progression of prostate cancer. J Transl Med 19:12 (2021). PubMed: 33407592
  • Wu Y  et al. Cyclophilin A regulates secretion of tumour-derived extracellular vesicles. Transl Oncol 14:101112 (2021). PubMed: 33984826
  • Yu J  et al. BMI-1 promotes invasion and metastasis in endometrial adenocarcinoma and is a poor prognostic factor. Oncol Rep 43:1630-1640 (2020). PubMed: 32323819
  • Zhou G  et al. Cyclophilin A contributes to aortopathy induced by postnatal loss of smooth muscle TGFBR1. FASEB J 33:11396-11410 (2019). PubMed: 31311317

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