Anti-Cyclophilin A抗体(ab3563)
Key features and details
- Rabbit polyclonal to Cyclophilin A
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cyclophilin A抗体
参阅全部 Cyclophilin A 一抗 -
描述
兔多克隆抗体to Cyclophilin A -
宿主
Rabbit -
特异性
Detects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Cat, Pig, Chimpanzee, Rhesus monkey, African green monkey
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免疫原
Recombinant full length protein corresponding to Human Cyclophilin A. Purified recombinant Human Cyclophilin A.
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阳性对照
- WB: CyPA; A549, U-87 MG, Jurkat cells; NIH/3T3, HeLa, mouse lung, colon and stomach tissue; HepG2, K562. ICC/IF: A431, HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
IgG fraction -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (2) |
1/300.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/300. |
靶标
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功能
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
序列相似性
Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5478 Human
- Entrez Gene: 268373 Mouse
- Omim: 123840 Human
- SwissProt: P62937 Human
- SwissProt: P17742 Mouse
- Unigene: 356331 Human
- Unigene: 598115 Human
- Unigene: 5246 Mouse
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别名
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)
Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized A431 cells staining Cyclophilin A with ab3563 at 1/250 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red Rhodamine Phalloidin, and the nuclear counterstain was SlowFade® Gold Antifade Mountant with DAPI (blue). Negative control (e) is where no primary antibody was used.
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)Immunocytochemistry analysis of formalin-fixed 0.1% Triton X-100 permeabilized HeLa cells staining Cyclophilin A with ab3563 at 1/200 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red phalloidin, and the nuclear counterstain was DAPI (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin A antibody (ab3563)Immunocytochemistry analysis of formalin-fixed 0.1% Triton X-100 permeabilized A431 cells staining Cyclophilin A with ab3563 at 1/200 dilution and DyLight-conjugated secondary antibody (green). F-actin was stained with red phalloidin, and the nuclear counterstain was DAPI (blue).
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Western blot - Anti-Cyclophilin A antibody (ab3563)All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/100 dilution
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : mouse lung tissue lysate
Lane 4 : mouse colon tissue lysate
Lane 5 : mouse stomach tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate at 1/2500 dilution
Developed using the ECL technique. -
Western blot - Anti-Cyclophilin A antibody (ab3563)All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/3000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, at 1/4000 dilution
Developed using the ECL technique. -
Western blot - Anti-Cyclophilin A antibody (ab3563)ab3563 at 1/300 dilution detecting 0.5ug recombinant human cyclophilin A by Western Blot (ECL).
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Western blot - Anti-Cyclophilin A antibody (ab3563)All lanes : Anti-Cyclophilin A antibody (ab3563) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : NIH-3T3
Lysates/proteins at 25 µg per lane.
Observed band size: 18 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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Datasheet download
文献 (11)
ab3563 被引用在 11 文献中.
- Storck SE et al. Brain endothelial LRP1 maintains blood-brain barrier integrity. Fluids Barriers CNS 18:27 (2021). PubMed: 34147102
- Francis AC et al. HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains. Nat Commun 11:3505 (2020). PubMed: 32665593
- Ma-Lauer Y et al. Influences of cyclosporin A and non-immunosuppressive derivatives on cellular cyclophilins and viral nucleocapsid protein during human coronavirus 229E replication. Antiviral Res 173:104620 (2020). PubMed: 31634494
- Cowman S et al. Decrease of Nibrin expression in chronic hypoxia is associated with hypoxia-induced chemoresistance in some brain tumour cells. BMC Cancer 19:300 (2019). PubMed: 30943920
- Francis AC & Melikyan GB Single HIV-1 Imaging Reveals Progression of Infection through CA-Dependent Steps of Docking at the Nuclear Pore, Uncoating, and Nuclear Transport. Cell Host Microbe 23:536-548.e6 (2018). PubMed: 29649444
