Anti-Cyclophilin 40抗体(ab226415)
Key features and details
- Rabbit polyclonal to Cyclophilin 40
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Cyclophilin 40抗体
参阅全部 Cyclophilin 40 一抗 -
描述
兔多克隆抗体to Cyclophilin 40 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Gorilla
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免疫原
Synthetic peptide within Human Cyclophilin 40 aa 175-225. The exact sequence is proprietary.
Database link: Q08752 -
阳性对照
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226415 was affinity purified using an epitope specific to Cyclophilin 40 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226415于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
|
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| IP |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 41 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 3 TPR repeats. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 737256 Chimpanzee
- Entrez Gene: 101152504 Gorilla
- Entrez Gene: 5481 Human
- Entrez Gene: 67738 Mouse
- Omim: 601753 Human
- SwissProt: Q08752 Human
- SwissProt: Q9CR16 Mouse
- Unigene: 183958 Human
see all -
别名
- 40 kDa peptidyl prolyl cis trans isomerase antibody
- 40 kDa peptidyl prolyl cis trans isomerase D antibody
- 40 kDa peptidyl-prolyl cis-trans isomerase antibody
see all
图片
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Western blot - Anti-Cyclophilin 40 antibody (ab226415)All lanes : Anti-Cyclophilin 40 antibody (ab226415) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 3 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Cyclophilin 40 antibody (ab226415)Cyclophilin 40 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226415 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226415 at 0.4 µg/ml.
Lane 1: ab226415 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226415 尚未被引用在任何文献中。