Anti-Cyclin T1抗体(ab176702)
Key features and details
- Rabbit polyclonal to Cyclin T1
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cyclin T1抗体
参阅全部 Cyclin T1 一抗 -
描述
兔多克隆抗体to Cyclin T1 -
宿主
Rabbit -
经测试应用
适用于: IP, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human Cyclin T1 aa 625-675. The exact sequence is proprietary.
Sequence:FSQPSCKTRVPHSKLDKGPTGANGHNTTQTIDYQDTVNMLHSLLSAQGVQ P
Database link: O60563 -
阳性对照
- WB: HeLa and 293T whole cell lysates; IP: HeLa whole cell lysate; IHC-P: Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176702于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
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| IHC-P |
1/1000.
|
| 说明 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 81 kDa. |
|
IHC-P
1/1000. |
靶标
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功能
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the cyclin family. Cyclin C subfamily. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 450146 Chimpanzee
- Entrez Gene: 904 Human
- Omim: 143055 Human
- SwissProt: Q8HXN7 Chimpanzee
- SwissProt: O60563 Human
- Unigene: 279906 Human
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别名
- CCN T1 antibody
- CCNT antibody
- CCNT 1 antibody
see all
图片
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Western blot - Anti-Cyclin T1 antibody (ab176702)All lanes : Anti-Cyclin T1 antibody (ab176702) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Predicted band size: 81 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Cyclin T1 antibody (ab176702)Detection of Cyclin T1 by Western Blot of Immunprecipitate.
Lane 1: ab176702 at 0.04µg/ml labeling Cyclin T1 in HeLa whole cell lysate immunoprecipitated using ab176702 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 3 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin T1 antibody (ab176702)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Cyclin T1 with ab 176702 at 1/1000 dilution (1 µg/mL).
数据表及文件
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Datasheet download
文献 (1)
ab176702 被引用在 1 文献中.
- Mück F et al. DDX6 transfers P-TEFb kinase to the AF4/AF4N (AFF1) super elongation complex. Am J Blood Res 6:28-45 (2016). PubMed: 27679741
