重组Anti-Cyclin E2抗体[E142] (ab32103)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E142] to Cyclin E2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cyclin E2抗体[E142]
参阅全部 Cyclin E2 一抗 -
描述
兔单克隆抗体[E142] to Cyclin E2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human Cyclin E2 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O96020 -
阳性对照
- Human breast carcinoma, HeLa cells lysates, Jurkat whole cell lysate (ab7899)
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E142 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32103于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (4) |
1/200. Predicted molecular weight: 45 kDa.
For unpurified version use at 1/500 |
ICC/IF | (1) |
1/50.
For unpurified use at 1/100 - 1/250 |
说明 |
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WB
1/200. Predicted molecular weight: 45 kDa. For unpurified version use at 1/500 |
ICC/IF
1/50. For unpurified use at 1/100 - 1/250 |
靶标
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相关性
The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 9134 Human
- Entrez Gene: 12448 Mouse
- Omim: 603775 Human
- SwissProt: O96020 Human
- SwissProt: Q7TMS8 Mouse
- SwissProt: Q9Z238 Mouse
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别名
- CCN E2 antibody
- CCNE 2 antibody
- CCNE2 antibody
see all
图片
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All lanes : Anti-Cyclin E2 antibody [E142] (ab32103) at 1/200 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse testis lysate
Lane 3 : Mouse thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa -
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin E2 with purified ab32103 at 1/50 dilution (5.2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-Cyclin E2 antibody [E142] (ab32103) at 1/500 dilution + HeLa cell lysate
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling Cyclin E2 with purified ab32103 at 1/2000. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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Immunofluorescent analysis of Cyclin E2 expression in HeLa cell culture using 1/100 ab32103.
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)This image is courtesy of an abreview by Kirk Mcmanus
ab32103 staining Cyclin E2 in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. Ab150081 (1/200) was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab32103 被引用在 25 文献中.
- Kim S et al. CDK4/6 initiates Rb inactivation and CDK2 activity coordinates cell-cycle commitment and G1/S transition. Sci Rep 12:16810 (2022). PubMed: 36207346
- Moyama C et al. Stat5b inhibition blocks proliferation and tumorigenicity of glioblastoma stem cells derived from a de novo murine brain cancer model. Am J Cancer Res 12:1129-1142 (2022). PubMed: 35411230
- Li Y et al. miRNA-128 modulates bone neoplasms cells proliferation and migration through the WNT/ß-catenin and EMT signal pathways. J Orthop Surg Res 16:71 (2021). PubMed: 33472642
- Hu T et al. RFC2, a direct target of miR-744, modulates the cell cycle and promotes the proliferation of CRC cells. J Cell Physiol 235:8319-8333 (2020). PubMed: 32239691
- Liang Y et al. Exosomal microRNA-144 from bone marrow-derived mesenchymal stem cells inhibits the progression of non-small cell lung cancer by targeting CCNE1 and CCNE2. Stem Cell Res Ther 11:87 (2020). PubMed: 32102682