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重组RabMAb

重组Anti-Cyclin E1抗体[EPR25394-23] - BSA and Azide free (ab317437)

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Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
  • Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
  • Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
  • Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
  • Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25394-23] to Cyclin E1 - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Cyclin E1抗体[EPR25394-23] - BSA and Azide free
    参阅全部 Cyclin E1 一抗

  • 描述

    兔单克隆抗体[EPR25394-23] to Cyclin E1 - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    This antibody does not cross-react with overexpressed mouse Cyclin E2 (#Q9Z238) by western blot.

  • 经测试应用

    适用于: WB, ICC/IFmore details
    不适用于: Flow Cyt (Intra),IHC-P or IP

  • 种属反应性

    与反应: Mouse, Rat
    不与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Untreated NIH/3T3, NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate, NIH/3T3 transfected with siRNA specifically targeting Cyclin E1, NIH/3T3 treated with 10 µg/ml aphidicolin for 24 hours whole, Mouse spleen, Mouse placenta, mouse lung, Mouse testis, Rat testis, Rat spleen,293T transfected with a mouse Cyclin E1 expression vector containing a His-tag ICC/IF: NIH/3T3 cells.

  • 常规说明

    ab317437 is the carrier-free version of ab317436

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317437于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
ICC/IF
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
ICC/IF
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt (Intra),IHC-P or IP.

靶标

  • 功能

    Essential for the control of the cell cycle at the G1/S (start) transition.

  • 组织特异性

    Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

  • 序列相似性

    Belongs to the cyclin family. Cyclin E subfamily.

  • 翻译后修饰

    Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CCNE antibody
      • Ccne1 antibody
      • CCNE1_HUMAN antibody
      • cyclin E variant ex5del antibody
      • cyclin E variant ex7del antibody
      • Cyclin E1 antibody
      • Cyclin Es antibody
      • Cyclin Et antibody
      • CyclinE antibody
      • G1/S specific cyclin E antibody
      • G1/S-specific cyclin-E1 antibody
      see all

    图片

    • Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
      Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
      All lanes : Anti-Cyclin E1 antibody [EPR25394-23] (ab317436) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate
      Lane 2 : NIH/3T3 transfected with siRNA specifically targeting Cyclin E1 whole cell lysate
      Lane 3 : Untreated NIH/3T3 whole cell lysate
      Lane 4 : NIH/3T3 treated with 10 µg /ml aphidicolin for 24 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 47 kDa
      Observed band size: 100,50 kDa why is the actual band size different from the predicted?



      This data was developed using ab317436, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression of Cyclin E1 is upregulated in response to aphidicolin treatment (PMID: 29995582).

      The identity of the higher molecular weight band at approximately 100 kDa may represent multi-ubiquitylated Cyclin E1 (PMID: 23109421).  This has not been confirmed with additional tests.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

      Exposure time: Lanes 1-2: 180 seconds, lanes 3-4: 103 seconds

    • Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
      Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
      All lanes : Anti-Cyclin E1 antibody [EPR25394-23] (ab317436) at 1/1000 dilution

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Mouse placenta tissue lysate
      Lane 3 : Mouse testis tissue lysate
      Lane 4 : Mouse lung tissue lysate
      Lane 5 : Mouse kidney tissue lysate
      Lane 6 : Mouse heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 47 kDa
      Observed band size: 100,50 kDa why is the actual band size different from the predicted?



      This data was developed using ab317436, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression:  lung, heart, kidney (PMID: 9840943, PMID: 11687642).

      The identity of the higher molecular weight band at approximately 100 kDa may represent multi-ubiquitylated Cyclin E1 (PMID: 23109421).  This has not been confirmed with additional tests.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

      Exposure time: Lane 1: 70 seconds, lanes 2-6: 180 seconds

    • Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
      Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
      All lanes : Anti-Cyclin E1 antibody [EPR25394-23] (ab317436) at 1/1000 dilution

      Lane 1 : Rat testis tissue lysate at 20 µg
      Lane 2 : Rat spleen tissue lysate at 20 µg
      Lane 3 : Rat heart tissue lysate
      Lane 4 : Rat kidney tissue lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 47 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab317436, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: heart, kidney (PMID: 9840943, PMID: 11687642).

      Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
      Western blot - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
      All lanes : Anti-Cyclin E1 antibody [EPR25394-23] (ab317436) at 1/1000 dilution

      Lane 1 : 293T cells transfected with an empty vector containing a His-tag, whole cell lysate
      Lane 2 : 293T cells transfected with a mouse Cyclin E1 expression vector containing a His-tag, whole cell lysate
      Lane 3 : 293T cells transfected with a mouse Cyclin E2 expression vector containing a His-tag, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 47 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      This data was developed using ab317436, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This antibody does not cross-react with mouse Cyclin E2.

      In Western blot, Anti-Vinculin antibody [EPR8185]  (ab129002) staining at 1/10000 dilution.

      In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade  (ab213204) staining at 1/5000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)

      This data was developed using ab317436, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Cyclin E1 with ab317436 at 1/500 (1.004 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

      Confocal image showing nuclear staining in NIH/3T3 cell line (shown in green), and decreased staining in cells during mitosis (indicated by arrowhead). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
      Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

    • Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)
      Anti-Cyclin E1 antibody [EPR25394-23] - BSA and Azide free (ab317437)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317437？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317437 尚未被引用在任何文献中。

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