Anti-Cyclin D2抗体- N-terminal (ab226972)
Key features and details
- Rabbit polyclonal to Cyclin D2 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cyclin D2抗体- N-terminal
参阅全部 Cyclin D2 一抗 -
描述
兔多克隆抗体to Cyclin D2 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Cyclin D2 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P30279 -
阳性对照
- WB: RD whole cell extract. IHC-P: Human cervical carcinoma tissue. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226972于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
|
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| IHC-P |
1/100 - 1/1000.
|
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| ICC/IF |
1/100 - 1/1000.
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| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
|
IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
靶标
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功能
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
序列相似性
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain. -
细胞定位
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. - Information by UniProt
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数据库链接
- Entrez Gene: 894 Human
- Omim: 123833 Human
- SwissProt: P30279 Human
- Unigene: 376071 Human
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别名
- CCND 2 antibody
- ccnd2 antibody
- CCND2_HUMAN antibody
see all
图片
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Western blot - Anti-Cyclin D2 antibody - N-terminal (ab226972)Anti-Cyclin D2 antibody - N-terminal (ab226972) at 1/1000 dilution + RD whole cell extract at 30 µg
Predicted band size: 33 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D2 antibody - N-terminal (ab226972)Paraffin-embedded human cervical carcinoma tissue stained for Cyclin D2 using ab226972 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D2 antibody - N-terminal (ab226972)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Cyclin D2 (green) using ab226972 at 1/500 dilution in ICC/IF. Nuclear counterstained with Hoechst 33342.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab226972 被引用在 4 文献中.
- Chang SC et al. Human FBXL8 Is a Novel E3 Ligase Which Promotes BRCA Metastasis by Stimulating Pro-Tumorigenic Cytokines and Inhibiting Tumor Suppressors. Cancers (Basel) 12:N/A (2020). PubMed: 32784654
- Liu B et al. A Novel Androgen-Induced lncRNA FAM83H-AS1 Promotes Prostate Cancer Progression via the miR-15a/CCNE2 Axis. Front Oncol 10:620306 (2020). PubMed: 33614501
- Yu G et al. lncRNA TUG1 Promotes Cisplatin Resistance by Regulating CCND2 via Epigenetically Silencing miR-194-5p in Bladder Cancer. Mol Ther Nucleic Acids 16:257-271 (2019). PubMed: 30925453
- He X et al. miR-4317 suppresses non-small cell lung cancer (NSCLC) by targeting fibroblast growth factor 9 (FGF9) and cyclin D2 (CCND2). J Exp Clin Cancer Res 37:230 (2018). PubMed: 30227870