Anti-Cyclin D1 (phospho T286)抗体(ab62151)
Key features and details
- Rabbit polyclonal to Cyclin D1 (phospho T286)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cyclin D1 (phospho T286)抗体
参阅全部 Cyclin D1 一抗 -
描述
兔多克隆抗体to Cyclin D1 (phospho T286) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human Cyclin D1 aa 200-300 (phospho T286).
Database link: P24385 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62151于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/2000. Detects a band of approximately 31 kDa (predicted molecular weight: 33 kDa). |
靶标
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功能
Essential for the control of the cell cycle at the G1/S (start) transition. -
疾病相关
Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. -
序列相似性
Belongs to the cyclin family. Cyclin D subfamily. -
翻译后修饰
Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 595 Human
- Entrez Gene: 12443 Mouse
- Omim: 168461 Human
- SwissProt: P24385 Human
- SwissProt: P25322 Mouse
- Unigene: 523852 Human
- Unigene: 667996 Human
- Unigene: 273049 Mouse
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别名
- AI327039 antibody
- B cell CLL/lymphoma 1 antibody
- B cell leukemia 1 antibody
see all
图片
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Western blot - Anti-Cyclin D1 (phospho T286) antibody (ab62151)All lanes : Anti-Cyclin D1 (phospho T286) antibody (ab62151) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from Jurkat cells treated with EGF (200ng/ml, 30mins) with immunizing phosphopeptide
Predicted band size: 33 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab62151 被引用在 6 文献中.
- Li Y et al. Eukaryotic initiation factor 3b regulates the development and progression of breast cancer. Am J Cancer Res 13:2188-2200 (2023). PubMed: 37293180
- Liu H et al. FBXL16 Promotes Endometrial Progesterone Resistance via PP2AB55α /Cyclin D1 Axis in Ishikawa. J Immunol Res 2022:7372202 (2022). PubMed: 36106050
- Guo Z et al. Essential meiotic structure-specific endonuclease1 (EME1) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway. Bioengineered 12:9869-9884 (2021). PubMed: 34719326
- Chen H et al. CDK4 protein is degraded by anaphase-promoting complex/cyclosome in mitosis and reaccumulates in early G1 phase to initiate a new cell cycle in HeLa cells. J Biol Chem 292:10131-10141 (2017). PubMed: 28446612
- Sun X et al. Let-7: a regulator of the ERa signaling pathway in human breast tumors and breast cancer stem cells. Oncol Rep 29:2079-87 (2013). PubMed: 23467929
- Biggar KK & Storey KB Evidence for cell cycle suppression and microRNA regulation of cyclin D1 during anoxia exposure in turtles. Cell Cycle 11:1705-13 (2012). WB . PubMed: 22510561