重组Anti-Cyclin D1抗体[rCCND1/4752] (ab273608)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [rCCND1/4752] (ab273608)](https://www.abcam.cn/ps/products/273/ab273608/Images/ab273608-393844-AntiCyclin-D1-antibody-rCCND1-4752-immunohistochemistry-mantle-cell-lymphoma-human.JPG "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [rCCND1/4752] (ab273608)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [rCCND1/4752] to Cyclin D1
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cyclin D1抗体[rCCND1/4752]
参阅全部 Cyclin D1 一抗 -
描述
小鼠单克隆抗体[rCCND1/4752] to Cyclin D1 -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Cyclin D1 aa 200-300.
Database link: P24385 -
阳性对照
- IHC-P: Human mantle cell lymphoma.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
rCCND1/4752 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273608于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Essential for the control of the cell cycle at the G1/S (start) transition. -
疾病相关
Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. -
序列相似性
Belongs to the cyclin family. Cyclin D subfamily. -
翻译后修饰
Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 595 Human
- Omim: 168461 Human
- SwissProt: P24385 Human
- Unigene: 523852 Human
- Unigene: 667996 Human
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别名
- AI327039 antibody
- B cell CLL/lymphoma 1 antibody
- B cell leukemia 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [rCCND1/4752] (ab273608)
Paraffin embedded human mantle cell lymphoma tissue stained for Cyclin D1 using ab273608 at 2 µg/ml in immunohistochemical analysis.
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![Anti-Cyclin D1 antibody [rCCND1/4752] (ab273608)](https://www.abcam.cn/ps/products/273/ab273608/Images/ab273608-2-benefits-of-recombinant-antibodies.png)
Anti-Cyclin D1 antibody [rCCND1/4752] (ab273608)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab273608 被引用在 1 文献中.
- Liu Y et al. High-Concentration Metformin Reduces Oxidative Stress Injury and Inhibits the Growth and Migration of Clear Cell Renal Cell Carcinoma. Comput Math Methods Med 2022:1466991 (2022). PubMed: 35592685