Anti-Cyclin D1抗体(ab226977)
Key features and details
- Rabbit polyclonal to Cyclin D1
- Suitable for: WB, IP, ICC/IF, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cyclin D1抗体
参阅全部 Cyclin D1 一抗 -
描述
兔多克隆抗体to Cyclin D1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IF, Sandwich ELISAmore details -
种属反应性
与反应: Human, Recombinant fragment
预测可用于: Mouse, Rat, Cow, Dog, Rhesus monkey
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免疫原
Recombinant full length protein corresponding to Human Cyclin D1.
Database link: P24385 -
阳性对照
- WB: HeLa, MCF7 and MDA-MB-231 whole cell extracts. IP: MCF7 whole cell extract. ICC/IF: SK-N-SH cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226977于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/10000. Predicted molecular weight: 34 kDa.
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| IP |
1/100 - 1/500.
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| ICC/IF |
1/100 - 1/1000.
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| Sandwich ELISA |
1/1000 - 1/10000.
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| 说明 |
|---|
|
WB
1/500 - 1/10000. Predicted molecular weight: 34 kDa. |
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IP
1/100 - 1/500. |
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ICC/IF
1/100 - 1/1000. |
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Sandwich ELISA
1/1000 - 1/10000. |
靶标
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功能
Essential for the control of the cell cycle at the G1/S (start) transition. -
疾病相关
Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. -
序列相似性
Belongs to the cyclin family. Cyclin D subfamily. -
翻译后修饰
Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 524530 Cow
- Entrez Gene: 449028 Dog
- Entrez Gene: 595 Human
- Entrez Gene: 12443 Mouse
- Entrez Gene: 58919 Rat
- Entrez Gene: 574320 Rhesus monkey
- Omim: 168461 Human
- SwissProt: Q2KI22 Cow
see all -
别名
- AI327039 antibody
- B cell CLL/lymphoma 1 antibody
- B cell leukemia 1 antibody
see all
图片
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Western blot - Anti-Cyclin D1 antibody (ab226977)All lanes : Anti-Cyclin D1 antibody (ab226977) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Cyclin D1 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 34 kDa12% SDS-PAGE gel.
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Western blot - Anti-Cyclin D1 antibody (ab226977)All lanes : Anti-Cyclin D1 antibody (ab226977) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 34 kDa12% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody (ab226977)4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells labeling Cyclin D1 (green) using ab226977 at 1/500 dilution in ICC/IF.
Counterstain: Cytoskeleton marker beta Tubulin 3/ Tuj1 antibody.
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Immunoprecipitation - Anti-Cyclin D1 antibody (ab226977)Cyclin D1 was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 µg ab226977. Western blot was performed from the immunoprecipitate using ab226977. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in MCF7 whole cell extract.
Lane 2: ab226977 IP in MCF7 whole cell extract.
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Sandwich ELISA - Anti-Cyclin D1 antibody (ab226977)Sandwich ELISA of recombinant full-length Cyclin D1 protein using ab226977 at 5μg/mL.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (119)
ab226977 被引用在 119 文献中.
- Jiang L et al. REG3A promotes proliferation and DDP resistance of ovarian cancer cells by activating the PI3K/Akt signaling pathway. Environ Toxicol 39:85-96 (2024). PubMed: 37665173
- Wang S et al. Knockdown of circ_0004585 enhances the chemosensitivity of colorectal cancer cells to 5-fluorouracil via the miR-874-3p/CCND1 axis. Histol Histopathol 38:99-112 (2023). PubMed: 35900059
- Liu T et al. Flavonoid extract Kushenol a exhibits anti-proliferative activity in breast cancer cells via suppression of PI3K/AKT/mTOR pathway. Cancer Med 12:1643-1654 (2023). PubMed: 35789211
- Karamichos D et al. Collagen Crosslinking for Keratoconus: Cellular Signaling Mechanisms. Biomolecules 13:N/A (2023). PubMed: 37189443
- Sun Y et al. Kremen2 drives the progression of non-small cell lung cancer by preventing SOCS3-mediated degradation of EGFR. J Exp Clin Cancer Res 42:140 (2023). PubMed: 37270563