Anti-Cyclin B1 (phospho S147)抗体(ab131502)
Key features and details
- Rabbit polyclonal to Cyclin B1 (phospho S147)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cyclin B1 (phospho S147)抗体
参阅全部 Cyclin B1 一抗 -
描述
兔多克隆抗体to Cyclin B1 (phospho S147) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Cyclin B1 aa 145-149 (phospho S147) conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:AFSpDV
Database link: P14635 -
阳性对照
- HeLa cells, MDA cells treated with anisomycin, Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab131502 was purified by epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131502于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 48 kDa.
|
|
| IHC-P |
1/50 - 1/100.
|
|
| ICC/IF |
1/100 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 48 kDa. |
|
IHC-P
1/50 - 1/100. |
|
ICC/IF
1/100 - 1/200. |
靶标
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功能
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
序列相似性
Belongs to the cyclin family. Cyclin AB subfamily. -
发展阶段
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
翻译后修饰
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
细胞定位
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Entrez Gene: 25203 Rat
- Omim: 123836 Human
- SwissProt: P14635 Human
- SwissProt: P24860 Mouse
- SwissProt: P30277 Rat
- Unigene: 23960 Human
see all -
别名
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
图片
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Western blot - Anti-Cyclin B1 (phospho S147) antibody (ab131502)All lanes : Anti-Cyclin B1 (phospho S147) antibody (ab131502) at 1/500 dilution
Lane 1 : Untreated MDA cell lysate
Lane 2 : Lysate of MDA cells treated with anisomycin
Predicted band size: 48 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 (phospho S147) antibody (ab131502)Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131502 at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 (phospho S147) antibody (ab131502)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelled with ab131502 at 1/50 dilution (left) and ab131502 preincubated with blocking peptide (right).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab131502 被引用在 1 文献中.
- Han L et al. Lamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei and Myocardial Regeneration. Dev Cell 53:42-59.e11 (2020). PubMed: 32109383