Anti-Cyclin B1 (phospho S126)抗体(ab55184)
Key features and details
- Rabbit polyclonal to Cyclin B1 (phospho S126)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Cyclin B1 (phospho S126)抗体
参阅全部 Cyclin B1 一抗 -
描述
兔多克隆抗体to Cyclin B1 (phospho S126) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Cyclin B1 (phospho S126). Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).
Database link: P14635 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab55184于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 48 kDa).
|
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| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 48 kDa). |
|
IHC-P
1/50 - 1/100. |
靶标
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功能
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
序列相似性
Belongs to the cyclin family. Cyclin AB subfamily. -
发展阶段
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
翻译后修饰
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
细胞定位
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Omim: 123836 Human
- SwissProt: P14635 Human
- SwissProt: P24860 Mouse
- Unigene: 23960 Human
- Unigene: 260114 Mouse
- Unigene: 380027 Mouse
see all -
别名
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
图片
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Western blot - Anti-Cyclin B1 (phospho S126) antibody (ab55184)All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab55184) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells
treated with EGF (200ng/ml, 15mins).
Lane 2 : extracts from NIH/3T3 cells
treated with EGF (200ng/ml, 15mins); ab36621 pre-incubated with the immunising phosphopeptide.
Predicted band size: 48 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 (phospho S126) antibody (ab55184)Immunohistochemical analysis of Cyclin B1 (phospho S126) expression in paraffin embedded human lung carcinoma tissue using ab55184 at 1/50 dilution (left). Right hand panel represents a negative control where ab55184 was pre-incubated with the immunising (blocking) phosphopeptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab55184 被引用在 9 文献中.
- Li B et al. Transcriptome analysis reveals a potential regulatory mechanism of the lnc-5423.6/IGFBP5 axis in the early stages of mouse thymic involution. Acta Biochim Biophys Sin (Shanghai) 55:548-560 (2023). PubMed: 37078747
- Gallo D et al. CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition. Nature 604:749-756 (2022). PubMed: 35444283
- Neizer-Ashun F et al. KRCC1, a modulator of the DNA damage response. Nucleic Acids Res 50:11028-11039 (2022). PubMed: 36243983
- Galarreta A et al. USP7 limits CDK1 activity throughout the cell cycle. EMBO J 40:e99692 (2021). PubMed: 33856059
- Lemmens B et al. DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation. Mol Cell 71:117-128.e3 (2018). PubMed: 30008317
- Müllers E et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). PubMed: 28345297
- Álvarez-Fernández M et al. Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals. Proc Natl Acad Sci U S A 110:17374-9 (2013). Mouse . PubMed: 24101512
- Zheng P & Dean J Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis. Proc Natl Acad Sci U S A 106:7473-8 (2009). ICC/IF ; Mouse . PubMed: 19376971
- Enomoto M et al. Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during G2/M transition. J Biol Chem 284:34223-30 (2009). WB ; Human . PubMed: 19837665