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Cell Biology Cell Cycle Cyclins

Anti-Cyclin B1 (phospho S126)抗体(ab3488)

  • Datasheet
  • SDS
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Western blot - Anti-Cyclin B1 (phospho S126) antibody (ab3488)

    Key features and details

    • Rabbit polyclonal to Cyclin B1 (phospho S126)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Cyclin B1 (phospho S126) antibody [EPR3670] (ab133439)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Cyclin B1 (phospho S126)抗体
      参阅全部 Cyclin B1 一抗
    • 描述

      兔多克隆抗体to Cyclin B1 (phospho S126)
    • 宿主

      Rabbit
    • 特异性

      Minimal reactivity is expected with the non-phosphorylated form of the protein.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Hamster, Dog, Chimpanzee
    • 免疫原

      Synthetic peptide corresponding to Human Cyclin B1 aa 120-131 (phospho S126).
      Sequence: ILVDTASpPSPME
      Database link: P14635

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明


      This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • Primary antibody说明

      This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cell Biology
      • Cell Cycle
      • Cyclins
      • Cell Biology
      • Cell Cycle
      • Cyclins
      • Cyclin B Family
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Cyclins
      • Cyclin B Family
      • Cancer
      • Cell cycle
      • Cyclins
      • Cyclin B family

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Cyclin B1 protein (ab128445)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3488于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
    说明
    WB
    1/100 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

    靶标

    • 功能

      Essential for the control of the cell cycle at the G2/M (mitosis) transition.
    • 序列相似性

      Belongs to the cyclin family. Cyclin AB subfamily.
    • 发展阶段

      Accumulates steadily during G2 and is abruptly destroyed at mitosis.
    • 翻译后修饰

      Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
    • 细胞定位

      Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
    • Target information above from: UniProt accession P14635 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 461835 Chimpanzee
      • Entrez Gene: 101836169 Hamster
      • Entrez Gene: 891 Human
      • Entrez Gene: 268697 Mouse
      • Entrez Gene: 25203 Rat
      • Omim: 123836 Human
      • SwissProt: P37882 Hamster
      • SwissProt: P14635 Human
      • SwissProt: P24860 Mouse
      • SwissProt: P30277 Rat
      • Unigene: 23960 Human
      • Unigene: 260114 Mouse
      • Unigene: 380027 Mouse
      • Unigene: 482545 Mouse
      • Unigene: 9232 Rat
      see all
    • 别名

      • CCNB 1 antibody
      • CCNB antibody
      • ccnb1 antibody
      • CCNB1_HUMAN antibody
      • Cyclin B1 antibody
      • G2 mitotic specific cyclin B1 antibody
      • G2/mitotic-specific cyclin-B1 antibody
      see all

    图片

    • Western blot - Anti-Cyclin B1 (phospho S126) antibody (ab3488)
      Western blot - Anti-Cyclin B1 (phospho S126) antibody (ab3488)
      All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : H23 whole cell lysate
      Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
      Lane 4 : Glioblastoma (T98G) cell lysate
      Lane 5 : B cell lymphoblast (Daudi) cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 48 kDa


      Exposure time: 20 seconds

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab3488?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3488 被引用在 1 文献中.

    • Müllers E  et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). PubMed: 28345297

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