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AB3488

Anti-Cyclin B1 (phospho S126)抗体

Anti-Cyclin B1 (phospho S126) antibody

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(1 Publication)

Rabbit Polyclonal Cyclin B1 phospho S126 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCNB1 phospho S126 aa 100-150.

查看别名

CCNB, CCNB1, G2/mitotic-specific cyclin-B1

1 Images
Western blot - Anti-Cyclin B1 (phospho S126) antibody (AB3488)
  • WB

Unknown

Western blot - Anti-Cyclin B1 (phospho S126) antibody (AB3488)

All lanes:

Western blot - Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

H23 whole cell lysate at 50 µg

Lane 3:

Hepatocarcinoma (Hep3b) cell lysate at 50 µg

Lane 4:

Glioblastoma (T98G) cell lysate at 50 µg

Lane 5:

B cell lymphoblast (Daudi) cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution

Predicted band size: 48 kDa

true

Exposure time: 20s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

Synthetic Peptide within Human CCNB1 phospho S126 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P14635

特异性

This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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您是否考虑以下替代产品？

AB133439

Anti-Cyclin B1 (phospho S126) antibody [EPR3670]

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为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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产品详情

This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
存储溶液
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Essential for the control of the cell cycle at the G2/M (mitosis) transition.
See full target information CCNB1 phospho S126
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 16:575-584 PubMed28345297

2017

Residual Cdk1/2 activity after DNA damage promotes senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Erik Müllers,Helena Silva Cascales,Kamila Burdova,Libor Macurek,Arne Lindqvist
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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