为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Cell Biology Cell Cycle Cyclins

Anti-Cyclin A2抗体[E23.1] (ab38)

  • Datasheet
  • SDS
Reviews (10)Q&A (8)References (51)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [E23.1] (ab38)
  • Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
  • Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)

Key features and details

  • Mouse monoclonal [E23.1] to Cyclin A2
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

你可能会感兴趣

试剂盒
Product image
beta Galactosidase Staining Kit (ab102534)
蛋白
Product image
Recombinant Human Cyclin A2 protein (ab126696)
二抗
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

查看更多相关产品

概述

  • 产品名称

    Anti-Cyclin A2抗体[E23.1]
    参阅全部 Cyclin A2 一抗
  • 描述

    小鼠单克隆抗体[E23.1] to Cyclin A2
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow
  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HEK293; U20S; PC12; NIH3T3. IHC-P: Human breast cancer tissue.
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    E23.1
  • 骨髓瘤

    Sp2
  • 同种型

    IgG2a
  • 轻链类型

    kappa
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cell Biology
    • Cell Cycle
    • Cyclins
    • Cyclin A Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cyclins
    • Cyclin A Family
    • Cancer
    • Cell cycle
    • Cyclins
    • Cyclin A family
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins

相关产品

  • Alternative Versions

    • Anti-Cyclin A2 antibody [E23.1] - BSA and Azide free (ab264078)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human Cyclin A2 protein (ab126696)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab38于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
Use a concentration of 5 µg/ml.
WB (3)
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa).
说明
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa).

靶标

  • 功能

    Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
  • 序列相似性

    Belongs to the cyclin family. Cyclin AB subfamily.
  • 发展阶段

    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • 细胞定位

    Nucleus. Cytoplasm. Cytoplasmic when associated with SCAPER.
  • Target information above from: UniProt accession P20248 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 281667 Cow
    • Entrez Gene: 890 Human
    • Entrez Gene: 12428 Mouse
    • Entrez Gene: 114494 Rat
    • Omim: 123835 Human
    • SwissProt: P30274 Cow
    • SwissProt: P20248 Human
    • SwissProt: P51943 Mouse
    • Unigene: 58974 Human
    • Unigene: 4189 Mouse
    see all
  • 别名

    • CCN1 antibody
    • CCNA antibody
    • Ccna2 antibody
    • CCNA2_HUMAN antibody
    • Cyclin A2 antibody
    • Cyclin-A antibody
    • Cyclin-A2 antibody
    see all

图片

  • Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
    Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
    All lanes : Anti-Cyclin A2 antibody [E23.1] (ab38) at 5 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 20 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [E23.1] (ab38)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [E23.1] (ab38)

    Immunohistochemical anaylsis of Cyclin A2 staining in Human breast cancer formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab38 (5 µg/ml) for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
    Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Cyclin A2 antibody [E23.1] (ab38) at 2 µg/ml

    Lane 1 : U2OS (Hu) whole cell lysate
    Lane 2 : 293T (Hu) whole cell lysate
    Lane 3 : Cos-7 (AGMk) whole cell lysate
    Lane 4 : CHO (Hm) whole cell lysate
    Lane 5 : PC12 (Rat) whole cell lysate
    Lane 6 : 3T3 (Ms) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-mouse at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?
    Additional bands at: 64 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 6 minutes


    The membrane was blocked with 5% milk for 1 hour at 21°C prior to incubation with the primary antibody for 12 hours at 4°C.

    See Abreview

  • Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
    Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)This image is courtesy of Julian Gannon, CRUK.
    Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD. Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD.

    See Abreview

实验方案

  • Mouse on Mouse staining protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (51)

发表研究结果有使用 ab38?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab38 被引用在 51 文献中.

  • Canu V  et al. Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation. Cell Death Differ 28:1493-1511 (2021). PubMed: 33230261
  • Zhao Y  et al. Leucine Improved Growth Performance, Muscle Growth, and Muscle Protein Deposition Through AKT/TOR and AKT/FOXO3a Signaling Pathways in Hybrid Catfish Pelteobagrus vachelli × Leiocassis longirostris. Cells 9:N/A (2020). PubMed: 32019276
  • Chen D  et al. LncRNA growth arrest-specific transcript 5 targets miR-21 gene and regulates bladder cancer cell proliferation and apoptosis through PTEN. Cancer Med 9:2846-2858 (2020). PubMed: 32069387
  • Tang W  et al. MicroRNA-29b-3p inhibits cell proliferation and angiogenesis by targeting VEGFA and PDGFB in retinal microvascular endothelial cells. Mol Vis 26:64-75 (2020). PubMed: 32165827
  • Wang P  et al. Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome development. J Clin Invest 130:4393-4407 (2019). PubMed: 31343991
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 18 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin A2 antibody [E23.1]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Permeabilization
No
Specification
MEF
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 06 2017

Western blot abreview for Anti-Cyclin A2 antibody [E23.1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T, MEL, K562 MCF7)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
22 µg
Specification
293T, MEL, K562 MCF7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 17 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin A2 antibody [E23.1]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH 3T3 (mouse embryonic fibroblast cell))
Permeabilization
Yes - tween-20
Specification
NIH 3T3 (mouse embryonic fibroblast cell)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 22 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cyclin A2 antibody [E23.1]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human breast adenocarcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Permeabilization
Yes - tween-20
Specification
human breast adenocarcinoma
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 22 2015

Western blot abreview for Anti-Cyclin A2 antibody [E23.1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Jurkat cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 22 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin A2 [E23.1] antibody

Poor
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Human Cell (Osteosarcoma)
Specification
Osteosarcoma
Permeabilization
Yes - triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 15 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin A antibody [E23.1]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (corneal endothelium)
Specification
corneal endothelium
Fixative
Formaldehyde
Permeabilization
Yes - 1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Zhiguo He

Verified customer

提交于 Nov 06 2012

Immunocytochemistry abreview for Anti-Cyclin A antibody [E23.1]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (corneal endothelial cells in vitro)
Specification
corneal endothelial cells in vitro
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Zhiguo He

Verified customer

提交于 Nov 06 2012

Question

Do you by chance have the non-hazardous declaration forms for ab9574 and ab87352 ready?

Read More

Abcam community

Verified customer

Asked on Nov 20 2012

Answer



The non hazardous SDS forms have been placed as 'SDS' PDF files available for download from the online product datasheets for ab9574 and ab87352 on Abcam's website.

Alternatively I have placed links to the PDF files below:

SDS for ab9574 https://www.abcam.com/ps/Products/9/ab9574/Msds/ab9574_WPNA_EN.pdf

SDS for ab87352 https://www.abcam.com/ps/Products/87/ab87352/Msds/ab87352_WPNA_EN.pdf

Read More

Abcam Scientific Support

回复于 Nov 20 2012

Question

thank you to your help. I think that this antibody doesn't work and I check in your web site to see if you have another cyclin A1 antibody but you have only the antibody for Xenopus laevis. Can I have a credit note for having anotherantibody?
Best regards

Read More

Abcam community

Verified customer

Asked on Jul 09 2012

Answer

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More

Abcam Scientific Support

回复于 Jul 09 2012

1-10 of 18 Abreviews or Q&A

  •  向后
  • 1
  • 2
  • 向前 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2023 艾博抗(上海)贸易有限公司版权所有