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Anti-Cyclin A2抗体[E23.1] (ab38)

Reviews (10)Q&A (8)References (51)
Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [E23.1] (ab38)
  • Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
  • Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)

Key features and details

  • Mouse monoclonal [E23.1] to Cyclin A2
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-Cyclin A2抗体[E23.1]
    参阅全部 Cyclin A2 一抗

  • 描述

    小鼠单克隆抗体[E23.1] to Cyclin A2

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HEK293; U20S; PC12; NIH3T3. IHC-P: Human breast cancer tissue.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab38于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
Use a concentration of 5 µg/ml.
WB (3)
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa).
说明
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa).

靶标

图片

  • Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
    Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
    All lanes : Anti-Cyclin A2 antibody [E23.1] (ab38) at 5 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 20 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [E23.1] (ab38)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A2 antibody [E23.1] (ab38)

    Immunohistochemical anaylsis of Cyclin A2 staining in Human breast cancer formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab38 (5 µg/ml) for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
    Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Cyclin A2 antibody [E23.1] (ab38) at 2 µg/ml

    Lane 1 : U2OS (Hu) whole cell lysate
    Lane 2 : 293T (Hu) whole cell lysate
    Lane 3 : Cos-7 (AGMk) whole cell lysate
    Lane 4 : CHO (Hm) whole cell lysate
    Lane 5 : PC12 (Rat) whole cell lysate
    Lane 6 : 3T3 (Ms) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-mouse at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?
    Additional bands at: 64 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 6 minutes


    The membrane was blocked with 5% milk for 1 hour at 21°C prior to incubation with the primary antibody for 12 hours at 4°C.

    See Abreview

  • Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)
    Western blot - Anti-Cyclin A2 antibody [E23.1] (ab38)This image is courtesy of Julian Gannon, CRUK.
    Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD. Western Blot of Swiss 3T3 cells showing presence of cyclin A in rapidly dividing cells (1) but absent in quiesent cells (2). Recombinant cyclin A (3). Molecular weight markers 114, 98, 68, 57, 43 and 32 kD.

    See Abreview

文献 (51)

发表研究结果有使用 ab38？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab38 被引用在 51 文献中.

  • Canu V  et al. Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation. Cell Death Differ 28:1493-1511 (2021). PubMed: 33230261
  • Zhao Y  et al. Leucine Improved Growth Performance, Muscle Growth, and Muscle Protein Deposition Through AKT/TOR and AKT/FOXO3a Signaling Pathways in Hybrid Catfish Pelteobagrus vachelli × Leiocassis longirostris. Cells 9:N/A (2020). PubMed: 32019276
  • Chen D  et al. LncRNA growth arrest-specific transcript 5 targets miR-21 gene and regulates bladder cancer cell proliferation and apoptosis through PTEN. Cancer Med 9:2846-2858 (2020). PubMed: 32069387
  • Tang W  et al. MicroRNA-29b-3p inhibits cell proliferation and angiogenesis by targeting VEGFA and PDGFB in retinal microvascular endothelial cells. Mol Vis 26:64-75 (2020). PubMed: 32165827
  • Wang P  et al. Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome development. J Clin Invest 130:4393-4407 (2019). PubMed: 31343991
View all Publications for this product

客户评价及客户问答

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1-10 of 18 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin A2 antibody [E23.1]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Permeabilization
No
Specification
MEF
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 06 2017

Western blot abreview for Anti-Cyclin A2 antibody [E23.1]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T, MEL, K562 MCF7)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
22 µg
Specification
293T, MEL, K562 MCF7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 17 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin A2 antibody [E23.1]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH 3T3 (mouse embryonic fibroblast cell))
Permeabilization
Yes - tween-20
Specification
NIH 3T3 (mouse embryonic fibroblast cell)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 22 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cyclin A2 antibody [E23.1]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human breast adenocarcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Permeabilization
Yes - tween-20
Specification
human breast adenocarcinoma
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 22 2015

Western blot abreview for Anti-Cyclin A2 antibody [E23.1]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Jurkat cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 22 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin A2 [E23.1] antibody

 Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Human Cell (Osteosarcoma)
Specification
Osteosarcoma
Permeabilization
Yes - triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 15 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin A antibody [E23.1]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (corneal endothelium)
Specification
corneal endothelium
Fixative
Formaldehyde
Permeabilization
Yes - 1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Zhiguo He

Verified customer

提交于 Nov 06 2012

Immunocytochemistry abreview for Anti-Cyclin A antibody [E23.1]

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (corneal endothelial cells in vitro)
Specification
corneal endothelial cells in vitro
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Zhiguo He

Verified customer

提交于 Nov 06 2012

Question

Do you by chance have the non-hazardous declaration forms for ab9574 and ab87352 ready?

Read More
Answer



The non hazardous SDS forms have been placed as 'SDS' PDF files available for download from the online product datasheets for ab9574 and ab87352 on Abcam's website.

Alternatively I have placed links to the PDF files below:

SDS for ab9574 https://www.abcam.com/ps/Products/9/ab9574/Msds/ab9574_WPNA_EN.pdf

SDS for ab87352 https://www.abcam.com/ps/Products/87/ab87352/Msds/ab87352_WPNA_EN.pdf

Read More

Question

thank you to your help. I think that this antibody doesn't work and I check in your web site to see if you have another cyclin A1 antibody but you have only the antibody for Xenopus laevis. Can I have a credit note for having anotherantibody?
Best regards

Read More
Answer

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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1-10 of 18 Abreviews or Q&A

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