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Anti-Cy5抗体[CY5-15] (ab52061)

Reviews (1)Q&A (7)References (4)

Key features and details

  • Mouse monoclonal [CY5-15] to Cy5
  • Suitable for: Dot blot, ELISA, IP, ICC, IHC-FoFr, IHC-FrFl, Flow Cyt
  • Reacts with: Species independent
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Cy5抗体[CY5-15]

  • 描述

    小鼠单克隆抗体[CY5-15] to Cy5

  • 宿主

    Mouse

  • 经测试应用

    适用于: Dot blot, ELISA, IP, ICC, IHC-FoFr, IHC-FrFl, Flow Cytmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Full length protein corresponding to Cy5. A mixture of proteins labelled with Cy5.

  • 阳性对照

    • IHC-Free Floating: Rat brain tissue. Flow Cytometry: Purified human CD4+ cells.

  • 常规说明

    Monoclonal antibodies to Cy5 are an important tool for Cy5 signal amplification in assays where biomolecules are labelled with Cy5.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯化说明

    Purified Immunoglobulin

  • Primary antibody说明

    Monoclonal antibodies to Cy5 are an important tool for Cy5 signal amplification in assays where biomolecules are labelled with Cy5.

  • 克隆

    单克隆

  • 克隆编号

    CY5-15

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab52061于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Dot blot
Use a concentration of 1 - 2 µg/ml.
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration.
IHC-FrFl (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 24376610

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
Dot blot
Use a concentration of 1 - 2 µg/ml.
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration.
IHC-FrFl
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 24376610

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性

    Cy3 and Cy5 are reactive watersoluble fluorescent dyes of the cyanine dye family used for labelling proteins, peptides, DNA, RNA, and other biomolecules. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically cross-linked to nucleic acids or protein molecules. Thay are small, pH insensitive (between pH 3 and pH 10), soluble in aqueous solution and have tolerance to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. They are used in many different biological assays, including DNA microarrays, protein microarrays, two-dimensional protein analysis, fluorescence resonance energy transfer, and immunocytochemistry. Cy3 is excited maximally at 550 nm and emits maximally at 570 nm, in the green part of the spectrum. Cy5 is excited maximally at 649 nm and emits maximally at 670 nm, in the red part of the spectrum.

文献 (4)

发表研究结果有使用 ab52061？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52061 被引用在 4 文献中.

  • Schafer JR  et al. Education-dependent activation of glycolysis promotes the cytolytic potency of licensed human natural killer cells. J Allergy Clin Immunol 143:346-358.e6 (2019). PubMed: 30096390
  • Rossiello F  et al. DNA damage response inhibition at dysfunctional telomeres by modulation of telomeric DNA damage response RNAs. Nat Commun 8:13980 (2017). PubMed: 28239143
  • Bolós M  et al. Soluble Tau has devastating effects on the structural plasticity of hippocampal granule neurons. Transl Psychiatry 7:1267 (2017). IHC ; Mouse . PubMed: 29217824
  • Cox JH  et al. Antibody-mediated targeting of the orai1 calcium channel inhibits T cell function. PLoS One 8:e82944 (2013). Flow Cyt . PubMed: 24376610

客户评价及客户问答

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1-8 of 8 Abreviews or Q&A

Immunohistochemistry - Free Floating abreview for Anti-Cy5 antibody [CY5-15]

 Average
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (brain)
Specification
brain
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Gitte Toft

Verified customer

提交于 Nov 04 2010

Question

Hallo lieber Abcam-Wissenschaftstdienst,

ich habe folgende Problem. Ich benutze einen hausinternen Tracer um ein Protein auf Maus Gehirnschnitten nachzuweisen. Der Tracer ist mit dem Fluorophor Cy5.5 gekoppelt. Nun würde ich gerne dieses Signal amplifizieren, da es viel zu schwach ist. Ich habe folgenden Ansatz und würde gerne wissen was sie dazu sagen:

erster Schritt: hausinterner Tracer gekoppelt mit Cy5.5 bindet an mein nachzuweisendes Protein im Gehirn

(ab52061) mouse monoklonal

dritter Schritt: EXPOSE mouse specific AP detection IHC kit (ab94747)

Was meinen sie dazu?

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Answer



Leider sehe ich ein paar Probleme bei diesem Protokoll:

ab52061 wurde nicht getestet ob er auch Cy5.5 erkennt. Das Immunogen war eine Mischung aus Cy5 konjugierten Proteinen.

Falls ab52061 Cy5.5 erkennen würde, hätten wir ein Problem mit dem sekundären Antikörper, da ab52061 aus Maus stammt und der passende sekundäre dann auch endogene Antikörper im Gewebe erkennen würde.

Ich schlage deshalb vor einen Antikerer zu nehmen, der getestet ist für Cy5.5 und aus Kaninchen stammt benutzen. Leider haben wir keinen solchen Antiköper im Sortiment im Moment.

Vielleicht kann die Biocompare Datenbank Ihnen da weiterhelfen:

www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

Wenn Sie solch einen Antikörper finden, könnte dann eines der folgenden Kits eingesetzt werden:

ab94734

https://www.abcam.com/EXPOSE-Mouse-and-Rabbit-Specific-AP-red-Detection-IHC-Kit-ab94734.html (or use the following: https://www.abcam.com/EXPOSE-Mouse-and-Rabbit-Specific-AP-red-Detection-IHC-Kit-ab94734.html).

ab94737

https://www.abcam.com/EXPOSE-Rabbit-Specific-AP-red-Detection-IHC-Kit-ab94737.html (or use the following: https://www.abcam.com/EXPOSE-Rabbit-Specific-AP-red-Detection-IHC-Kit-ab94737.html).

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Question

Customer kindly contacted us regarding ab52061 cross reactivity against Cy5.5 or Cy7.

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Answer


Unfortunately, ab52061 has not been tested against either Cy5.5 nor Cy7. Based on the dissimilarity in chemical structure of Cy5.5 and Cy7 vs Cy5, it seems unlikely that ab52061 will recognize either Cy5.5 or Cy7.

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Question

I'm a neuroscience researcher working on neural anatomical tract tracing and imaging. My work is mainly about inject fluorescent tracers into animal brain in vivo and let the tracer undergo anterograde or retrograde transport, to label neural tissues. So one of the tracer which providing me very good quality is Alexa 647 (http://products.invitrogen.com/ivgn/product/D22914), from invitrogen. What is more complicated is i also want to check the tissue under electron microscope, so I will need to further treated the tissue (normally 80um brain slices) with OsO4 (osmium tetroxide) and embed it into durcupan. Then I will cut ultra-thin section out of it, typically in the range of 60˜80nm. So ideally i want to visulize Alexa 647 signal on those treated ultra-thin sections, but at this level normally the fluorescent was to a large extend killed or diminished. With some other tracer (eg:Texas Red or Lucifer Yellow), we tried commercial available antibody to target the tracer in those ultra-thin sections and re-visulize it, and sometimes works. However it seems there's very rare primary antibody which could targeting Alexa 647, on the market.

So I went across your product ab52060 and ab52061 (they seems developed to target Cy5), and found in their description they can also target Alexa 647. It will helps me a lot if you can give me some more information about these two antibodies, like how specific it can bind to Alexa 647 (preferably if you can give me some images or reference that tried this antibody on Alexa 647)? And do you think it can somehow works after the possible transformational change of the fluorophore from my treatment?

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Answer

Thank you for contacting us.

Unfortunately we do not have any images of product ab52061 and ab52060 with Alexa 647. Alexa 647 is based on the structure of Cy5 and therefore is structurally very similar. As our data sheet states this antibody cross-reacts with Alexa 647, it is likely this was tested during R&D stages.

The only reference that we are aware of for product ab52060 is:
Myosin-Va Mediates RNA Distribution In Primary Fibroblasts From Multiple Organs. Salerno, V.P., et al. Cell Motil. Cytoskeleton 65, 422-33, (2008)

We have not tested either antibody with denatured Cy5 or Alexa 647. If the structure is changed quite drastically I think it likely that the antibodies would not recognise the molecule. If the changes to the structure are minor there may be a chance of recognition occurring. As ab52060 has wider reactivity (Cy5 and Cy3 related molecules), this might be more likely than ab52061 to be tolerant to slight changes to target molecule structure.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Dear Madam, Dear Sir,

I would like to amplify a signal from Cy5.5 on tissue section. I was wondering if your anti-Cy5 (Anti-Cy5 antibody [CY5-15] (ab52061))can react with Cy5.5? if not, do you know any anti-Cy5.5?

Thank you a lot for your help,

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Answer

Thank you for your inquiry.

Unfortunately I am sorry to confirm we have no data or experimental information to verify whether ab52061 Cy5 antibody [CY5-15]cross reacts with Cy5.5. The Cy5 and Cy5.5 structures are quite similar and so the possibility of cross reactivity cannot be ruled out. However, we have not data to confirm or guarantee this.

I am sorry we also do not have any antibodies against Cy5.5 available in our catalog. Therefore, I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:

http://www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

I am sorry we do not have the product you require on this occasion. However, I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions.

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Question

I was wondering if this product will cross react with AF680.

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Answer

Thank you for contacting us.

Unfortunately we have no data or experimental information to verify whether product ab52061 (anti-Cy5) cross reacts with AF-680.

Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Will this antibody cross-react with Cy 5.5?

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Answer

Thank you for your inquiry.

Unfortunately we have no information on whether this antibody cross-reacts with Cy5.5. However looking at a comparison of the molecules that I have attached I believe that it is probably highly unlikely that it will cross-react as the molecules are significantly different.

I checked our catalogue and we do not carry an anti-Cy5.5 antibody.

I am sorry that this information could not be more helpful. Please contact us with any other questions.

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Question

Dear technical team,

Our customer is going to purchase ab52061 and her sample is a mixture of proteins which labelled with biotin, tamra, Cy5. So
She would like to know if it detects proteins labelled with Cy5 only.
Please check this.

Best regards

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Answer

Thank you and your customer for your inquiry.

I am happy to confirm that ab52061 will only bind to Cy5 and not to the Rhodamine related Tamra or to biotin.

This is tested and guaranteed by our Abpromise.

I wish your customer good luck with their research.

Read More

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