Anti-Cy3 / Cy5抗体[CY96] (ab52060)
Key features and details
- Mouse monoclonal [CY96] to Cy3 / Cy5
- Suitable for: Dot blot, ELISA, IP, ICC, ICC/IF
- Reacts with: Species independent
- Isotype: IgG2a
概述
-
产品名称
Anti-Cy3 / Cy5抗体[CY96] -
描述
小鼠单克隆抗体[CY96] to Cy3 / Cy5 -
宿主
Mouse -
经测试应用
适用于: Dot blot, ELISA, IP, ICC, ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
A mixture of proteins labelled with Cy3 or Cy5.
-
常规说明
Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
-
纯化说明
Purified Mouse Immunoglobulin -
Primary antibody说明
Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5. -
克隆
单克隆 -
克隆编号
CY96 -
同种型
IgG2a -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52060于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Dot blot |
Use a concentration of 1 - 2 µg/ml.
|
|
ELISA |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
|
ICC |
Use at an assay dependent concentration.
|
|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
说明 |
---|
Dot blot
Use a concentration of 1 - 2 µg/ml. |
ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
靶标
-
相关性
Cy3 and Cy5 are reactive watersoluble fluorescent dyes of the cyanine dye family used for labelling proteins, peptides, DNA, RNA, and other biomolecules. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically cross-linked to nucleic acids or protein molecules. Thay are small, pH insensitive (between pH 3 and pH 10), soluble in aqueous solution and have tolerance to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. They are used in many different biological assays, including DNA microarrays, protein microarrays, two-dimensional protein analysis, fluorescence resonance energy transfer, and immunocytochemistry. Cy3 is excited maximally at 550 nm and emits maximally at 570 nm, in the green part of the spectrum. Cy5 is excited maximally at 649 nm and emits maximally at 670 nm, in the red part of the spectrum.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (7)
ab52060 被引用在 7 文献中.
- Wang C et al. RasGRP1 promotes the acute inflammatory response and restricts inflammation-associated cancer cell growth. Nat Commun 13:7001 (2022). PubMed: 36385095
- Baltazar-Lara R et al. Growth Hormone (GH) Crosses the Blood-Brain Barrier (BBB) and Induces Neuroprotective Effects in the Embryonic Chicken Cerebellum after a Hypoxic Injury. Int J Mol Sci 23:N/A (2022). PubMed: 36232848
- Rodriguez FJ et al. Alternative lengthening of telomeres, ATRX loss and H3-K27M mutations in histologically defined pilocytic astrocytoma with anaplasia. Brain Pathol 29:126-140 (2019). PubMed: 30192422
- Rodriguez FJ et al. Telomere alterations in neurofibromatosis type 1-associated solid tumors. Acta Neuropathol Commun 7:139 (2019). PubMed: 31462295
- Palsgrove DN et al. Subependymal giant cell astrocytoma-like astrocytoma: a neoplasm with a distinct phenotype and frequent neurofibromatosis type-1-association. Mod Pathol N/A:N/A (2018). PubMed: 29973652
- Osterwald S et al. PML induces compaction, TRF2 depletion and DNA damage signaling at telomeres and promotes their alternative lengthening. J Cell Sci 128:1887-900 (2015). PubMed: 25908860
- Jeffery R et al. Identifying the origin and phenotype of cells in tumor xenografts. Methods Mol Biol 1070:235-45 (2014). PubMed: 24092445