Cy3 ® Anti-beta Tubulin抗体[TUB 2.1] (ab11309)
Key features and details
- Cy3 ® Mouse monoclonal [TUB 2.1] to beta Tubulin
- Suitable for: ICC/IF
- Reacts with: Chicken
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Isotype: IgG1
概述
-
产品名称
Cy3 ® Anti-beta Tubulin抗体[TUB 2.1]
参阅全部 beta Tubulin 一抗 -
描述
Cy3 ®小鼠单克隆抗体[TUB 2.1] to beta Tubulin -
宿主
Mouse -
偶联物
Cy3 ®. Ex: 552nm, Em: 565nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Chicken -
免疫原
Full length protein corresponding to beta Tubulin. Purified rat brain tubulin.
-
表位
Ab11309 specifically recognizes an epitope in the carboxy-terminal part of beta-tubulin (between amino acids 281-446). -
阳性对照
- Cultured chicken fibroblasts.
-
常规说明
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The product is prepared by conjugation of Cy3 to purified monoclonal anti-beta-tubulin antibody. The conjugate is purified by gel filtration to remove unbound Cy3 fluorophore. F/P Molar Ratio: (Cy3:Ab) 3 to 9.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading...
-
纯度
Proprietary Purification -
克隆
单克隆 -
克隆编号
TUB 2.1 -
同种型
IgG1 -
研究领域
相关产品
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11309于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (3) |
1/200.
|
说明 |
---|
ICC/IF
1/200. |
靶标
-
功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
组织特异性
Ubiquitously expressed with highest levels in spleen, thymus and immature brain. -
疾病相关
Cortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1 -
序列相似性
Belongs to the tubulin family. -
结构域
The highly acidic C-terminal region may bind cations such as calcium. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. -
细胞定位
Cytoplasm, cytoskeleton. - Information by UniProt
-
数据库链接
- Entrez Gene: 396254 Chicken
-
别名
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
see all
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (11)
ab11309 被引用在 11 文献中.
- Dong J et al. Ectopic expression of human TUBB8 leads to increased aneuploidy in mouse oocytes. Cell Discov 9:105 (2023). PubMed: 37875488
- Guo-Parke H et al. Altered Differentiation and Inflammation Profiles Contribute to Enhanced Innate Responses in Severe COPD Epithelium to Rhinovirus Infection. Front Med (Lausanne) 9:741989 (2022). PubMed: 35280870
- Kelly JN et al. Comprehensive single cell analysis of pandemic influenza A virus infection in the human airways uncovers cell-type specific host transcriptional signatures relevant for disease progression and pathogenesis. Front Immunol 13:978824 (2022). PubMed: 36268025
- Touzelet O et al. The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins. Mol Cell Proteomics 19:793-807 (2020). PubMed: 32075873
- Matamoros AJ et al. Knockdown of Fidgetin Improves Regeneration of Injured Axons by a Microtubule-Based Mechanism. J Neurosci 39:2011-2024 (2019). PubMed: 30647150