重组Anti-CXCR6抗体[EPR24686-113] (ab273116)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] (ab273116)](https://www.abcam.cn/ps/products/273/ab273116/Images/ab273116-1-anti-cxcr6-antibody-epr24686113-immunohistochemistry-human-diffuse-large-b-cell-lymphoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] (ab273116)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24686-113] to CXCR6
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CXCR6抗体[EPR24686-113]
参阅全部 CXCR6 一抗 -
描述
兔单克隆抗体[EPR24686-113] to CXCR6 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details
不适用于: IP or WB -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human diffuse large B lymphoma and Human tonsil tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR24686-113 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273116于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1. -
组织特异性
Expressed in lymphoid tissues and activated T cells. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10663 Human
- Omim: 605163 Human
- SwissProt: O00574 Human
- Unigene: 34526 Human
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别名
- bonzo antibody
- C X C chemokine receptor type 6 antibody
- C-X-C chemokine receptor type 6 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] (ab273116)
Immunohistochemical analysis of paraffin-embedded Human diffuse large B cell lymphoma tissue labelling CXCR6 with ab273116 at 1/1000 dilution (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous and cytoplasmic staining in human diffuse large B cell lymphoma. The section was incubated with ab273116 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] (ab273116)](https://www.abcam.cn/ps/products/273/ab273116/Images/ab273116-2-anti-cxcr6-antibody-epr24686113-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] (ab273116)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CXCR6 with ab273116 at 1/1000 dilution (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous and cytoplasmic staining on scattered immune cells of human tonsil. The section was incubated with ab273116 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] (ab273116)](https://www.abcam.cn/ps/products/273/ab273116/Images/ab273116-3-anti-cxcr6-antibody-epr24686113-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR6 antibody [EPR24686-113] (ab273116)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling CXCR6 with ab273116 at 1/1000 dilution (0.533 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human cerebrum (PMID: 9230441). The section was incubated with ab273116 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-CXCR6 antibody [EPR24686-113] (ab273116)](https://www.abcam.cn/ps/products/273/ab273116/Images/ab273116-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-CXCR6 antibody [EPR24686-113] (ab273116)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab273116 被引用在 3 文献中.
- Li X et al. Integrated single cell and bulk sequencing analysis identifies tumor reactive CXCR6+ CD8 T cells as a predictor of immune infiltration and immunotherapy outcomes in hepatocellular carcinoma. Front Oncol 13:1099385 (2023). PubMed: 37593098
- Piehl N et al. Cerebrospinal fluid immune dysregulation during healthy brain aging and cognitive impairment. Cell 185:5028-5039.e13 (2022). PubMed: 36516855
- Zhou H et al. Diagnostic Biomarkers and Immune Infiltration in Patients With T Cell-Mediated Rejection After Kidney Transplantation. Front Immunol 12:774321 (2021). PubMed: 35058922