重组Anti-CXCR4抗体[EPUMBR3] - BSA and Azide free (ab271934)
![Flow Cytometry (Intracellular) - Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934)](https://www.abcam.cn/ps/products/271/ab271934/Images/ab271934-6-ab181020333813anticxcr4antibodyepumbr3flowcytometry.jpg "Flow Cytometry (Intracellular) - Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPUMBR3] to CXCR4 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CXCR4抗体[EPUMBR3] - BSA and Azide free
参阅全部 CXCR4 一抗 -
描述
兔单克隆抗体[EPUMBR3] to CXCR4 - BSA and Azide free -
宿主
Rabbit -
特异性
This antibody recognizes only the non-phosphorylated C-terminus of CXCR4 (residues 341-352). Phosphorylation of S346/347 blocks antibody binding. PMID: 24154522, 25451233.
We recommend dephosphorylation of samples using lambda phosphatase treatment. Please refer to application notes.
Although some customers can get this ab to work in mouse and rat successfully, we cannot reproduce this in house in IHC so cannot guarantee it. We would recommend antibody Anti-CXCR4 antibody [UMB2] (ab124824) for use in mouse IHC.
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经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat whole cell lysate. ICC/IF: Jurkat and Ramos cells. IHC-P: Human small cell lung carcinoma tissue. Flow Cyt (intra): Jurkat cells.
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常规说明
ab271934 is the carrier-free version of ab181020.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPUMBR3 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271934于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
We recommend lambda protein phosphatase treatment of the membrane prior to primary antibody incubation (PMID 24154522). Use 800U for 1 hr at RT then rinse in wash buffer three times.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Boil slides seven times for five minutes each in pH 6 citrate buffer.
We recommend lambda protein phosphatase treatment prior to IHC processing (PMID 24154522). Use 800U for 1 hr at RT then rinse in PBS three times.
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| ICC/IF |
Use at an assay dependent concentration.
For unpurified use at 5 µg/mL.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa. We recommend lambda protein phosphatase treatment of the membrane prior to primary antibody incubation (PMID 24154522). Use 800U for 1 hr at RT then rinse in wash buffer three times.
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Boil slides seven times for five minutes each in pH 6 citrate buffer. We recommend lambda protein phosphatase treatment prior to IHC processing (PMID 24154522). Use 800U for 1 hr at RT then rinse in PBS three times.
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ICC/IF
Use at an assay dependent concentration. For unpurified use at 5 µg/mL.
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. -
组织特异性
Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. -
疾病相关
Defects in CXCR4 are a cause of WHIM syndrome (WHIM) [MIM:193670]; also known as warts, hypogammaglobulinemia, infections and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
结构域
The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity. -
翻译后修饰
Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.
Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S.
Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization.
O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity. -
细胞定位
Cell membrane. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. - Information by UniProt
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数据库链接
- Entrez Gene: 7852 Human
- Entrez Gene: 12767 Mouse
- Omim: 162643 Human
- SwissProt: P61073 Human
- SwissProt: P70658 Mouse
- Unigene: 593413 Human
- Unigene: 1401 Mouse
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别名
- C-X-C chemokine receptor type 4 antibody
- CD184 antibody
- CD184 antigen antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934)
Intracellular Flow Cytometry analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling CXCR4 with purified ab181020 at 1/200 dilution (10.23µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181020).
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![Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934)](https://www.abcam.cn/ps/products/271/ab271934/Images/ab271934-5-ab181020273052anticxcr4antibodyepumbr3immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934)Immunocytochemistry/Immunofluorescence analysis of Ramos (Human Burkitt's lymphoma cell line) labeling CXCR4 with purified ab181020 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181020). -
![Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934)](https://www.abcam.cn/ps/products/271/ab271934/Images/ab271934-2-ab181020252132anticxcr4antibodyepumbr3immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934)ab181020 stained Jurkat cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab181020 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181020). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934)](https://www.abcam.cn/ps/products/271/ab271934/Images/ab271934-1-ab181020214831anticxcr4antibodyepumbr3immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934) -
![Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934)](https://www.abcam.cn/ps/products/271/ab271934/Images/ab271934-7-benefits-of-recombinant-antibodies.png)
Anti-CXCR4 antibody [EPUMBR3] - BSA and Azide free (ab271934)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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