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AB61100

Anti-CXCR2/CD182 (phospho S347)抗体

Anti-CXCR2/CD182 (phospho S347) antibody

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(3 Publications)

Rabbit Polyclonal CXCR2 phospho S347 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CXCR2/CD182 pS347 aa 300 to C-terminus.

查看别名

CD182, IL8RB, CXCR2, C-X-C chemokine receptor type 2, CXC-R2, CXCR-2, CDw128b, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8 receptor type 2, IL-8R B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2/CD182 (phospho S347) antibody (AB61100)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2/CD182 (phospho S347) antibody (AB61100)

1/50-1/100 ab61100 staining phosphorilated CXCR2 on human breast carcinoma tissue, in the presence or absence of the immunizing phospopeptide.

Western blot - Anti-CXCR2/CD182 (phospho S347) antibody (AB61100)
  • WB

Supplier Data

Western blot - Anti-CXCR2/CD182 (phospho S347) antibody (AB61100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-IL-8Rβ (Phospho-Ser347) antibody.

All lanes were loaded with lysates from PMA-stimulated 3T3 cells.

Lane 2 : The membrane was incubated with anti-IL-8Rβ (Phospho-Ser347) antibody in the presence of phospho-IL-8Rβ peptide (blocking peptide).

All lanes:

Western blot - Anti-CXCR2/CD182 (phospho S347) antibody (ab61100) at 1/500 dilution

All lanes:

PMA-stimulated 3T3 cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody at 1/5000 dilution

Predicted band size: 41 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse

应用

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human CXCR2/CD182 pS347 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25025

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替代产品

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CXCR2 also called C-X-C chemokine receptor type 2 is a G protein-coupled receptor with a mass of approximately 53 kDa. It primarily binds to chemokines like CXCL1 CXCL2 CXCL3 CXCL5 CXCL6 CXCL7 and CXCL8 (also known as IL-8). CXCR2 is expressed in a variety of cells but is found especially in neutrophils where it plays a role in mediating immune response. Additionally it shows expression in some epithelial and endothelial cells contributing to its specialized functions in various tissues.
Biological function summary

CXCR2 plays significant roles in mediating inflammatory responses and is part of the chemokine receptor family. It functions by promoting chemotaxis of neutrophils to sites of inflammation infection or injury. This receptor operates with other chemokine receptors in the recruitment and migration of different immune cells helping coordinate an effective immune response. CXCR2 does not function alone and often interacts with other cell surface receptors and signaling molecules during immune responses.

Pathways

CXCR2 contributes prominently to the chemokine signaling pathway and the inflammatory response pathway. It interacts closely with proteins like CXCR1 another chemokine receptor to perform its functions. These interactions can activate downstream signaling cascades involving MAPK and PI3K pathways which are important for various cellular responses like proliferation survival and migration.

CXCR2 has associations with inflammatory diseases such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In these conditions excessive activation or expression of CXCR2 can lead to pathological inflammation. CXCR2 coordinates with proteins like IL-8 contributing to the chemotaxis of inflammatory cells in affected tissues and this coordination often exacerbates the inflammatory process in these diseases.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor (PubMed : 1891716). Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 8662698). Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2 (PubMed : 1891716). Involved in the homeostatic wound healing response to tissue injury, a multistep cascade that guides neutrophil migration to necrotic sites while avoiding collateral damage of healthy tissues. Signals intravascular neutrophil chemotaxis to the injury site.
See full target information CXCR2/CD182 pS347
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:862675 PubMed35592244

2022

CXC Chemokine Receptor 2 Accelerates Tubular Cell Senescence and Renal Fibrosis β-Catenin-Induced Mitochondrial Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Meng,Jiewu Huang,Xian Ling,Shan Zhou,Jingyan Wei,Mingsheng Zhu,Jinhua Miao,Weiwei Shen,Jiemei Li,Huiyun Ye,Hongxin Niu,Yunfang Zhang,Lili Zhou

Journal of vascular research 56:39-53 PubMed30995642

2019

Electrical Stimulation Directs Migration, Enhances and Orients Cell Division and Upregulates the Chemokine Receptors CXCR4 and CXCR2 in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Filipa Cunha,Ann M Rajnicek,Colin D McCaig

Cancer letters 308:91-9 PubMed21601983

2011

Mesenchymal stem cells promote mammary cancer cell migration in vitro via the CXCR2 receptor.

Applications

ICC/IF, WB

Species

Mouse, Mouse

Jennifer L Halpern,Amy Kilbarger,Conor C Lynch
View all publications
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