Anti-CXCL2抗体(ab9777)
Key features and details
- Rabbit polyclonal to CXCL2
- Suitable for: ELISA, Nucleotide Array, IHC-Fr, Sandwich ELISA, Neutralising, WB
- Reacts with: Rat
- Isotype: unknown
概述
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产品名称
Anti-CXCL2抗体
参阅全部 CXCL2 一抗 -
描述
兔多克隆抗体to CXCL2 -
宿主
Rabbit -
经测试应用
适用于: ELISA, Nucleotide Array, IHC-Fr, Sandwich ELISA, Neutralising, WBmore details -
种属反应性
与反应: Rat -
免疫原
Recombinant full length protein corresponding to Rat CXCL2 aa 1-100.
Database link: P30348 -
阳性对照
- WB: Recombinant rat CXCL2 protein (ab9778).
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常规说明
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
No preservative, sterile filtered -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| Nucleotide Array |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration. PubMed: 23776670
|
|
| Sandwich ELISA |
Use at an assay dependent concentration.
To detect Rat CXCL2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of ab9777 is required. This antigen affinity purified antibody, in conjunction with an Anti-Rat CXCL2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CXCL2. |
|
| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of rGRO beta/MIP-2 (100 ng/ml), a concentration of 3.0 - 5.0 µg/ml of this antibody is required. |
|
| WB |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
Nucleotide Array
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 23776670 |
|
Sandwich ELISA
Use at an assay dependent concentration. To detect Rat CXCL2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of ab9777 is required. This antigen affinity purified antibody, in conjunction with an Anti-Rat CXCL2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CXCL2. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of rGRO beta/MIP-2 (100 ng/ml), a concentration of 3.0 - 5.0 µg/ml of this antibody is required. |
|
WB
Use at an assay dependent concentration. |
靶标
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功能
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 114105 Rat
- SwissProt: P30348 Rat
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别名
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
see all
数据表及文件
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Datasheet download
文献 (7)
ab9777 被引用在 7 文献中.
- Iwamoto S et al. RGMa Signal in Macrophages Induces Neutrophil-Related Astrocytopathy in NMO. Ann Neurol 91:532-547 (2022). PubMed: 35167145
- Memedovski Z et al. Classical and Alternative Activation of Rat Microglia Treated with Ultrapure Porphyromonas gingivalis Lipopolysaccharide In Vitro. Toxins (Basel) 12:N/A (2020). PubMed: 32438602
- Pan T et al. Knocking down TRPM2 expression reduces cell injury and NLRP3 inflammasome activation in PC12 cells subjected to oxygen-glucose deprivation. Neural Regen Res 15:2154-2161 (2020). PubMed: 32394974
- Nguyen PNN et al. miR-524-5p of the primate-specific C19MC miRNA cluster targets TP53IPN1- and EMT-associated genes to regulate cellular reprogramming. Stem Cell Res Ther 8:214 (2017). WB . PubMed: 28962647
- Yu C et al. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun 6:8017 (2015). PubMed: 26281983
- Perino MG et al. Chemokine localization in bronchial angiogenesis. PLoS One 8:e66432 (2013). IHC-Fr ; Rat . PubMed: 23776670
- Shastry S & James LR Homocysteine-induced macrophage inflammatory protein-2 production by glomerular mesangial cells is mediated by PI3 Kinase and p38 MAPK. J Inflamm (Lond) 6:27 (2009). WB ; Rat . PubMed: 19781090
