AB199043

重组Anti-CXCL13抗体[EPR19259-147]

Name: CXCL13抗体[EPR19259-147] (ab199043)| Abcam中文官网
Brand: Abcam Limited
SKU: AB199043
Price: 3357 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-CXCL13 antibody [EPR19259-147]

RabMAb
Recombinant
了解详情

(2 Reviews)

(6 Publications)

Rabbit Recombinant Monoclonal BCA1 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 6 publications.

查看别名

Blc, Scyb13, Cxcl13, C-X-C motif chemokine 13, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR19259-147] (AB199043)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR19259-147] (AB199043)

Negative Control :

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling CXCL13 with ab199043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. No staining on mouse cerebral cortex (PMID : 17563386). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR19259-147] (AB199043)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CXCL13 with ab199043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic staining on mouse spleen (PMID : 17563386). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

不同偶联物与剂型 (1)

Carrier free

Anti-CXCL13 antibody [EPR19259-147] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19259-147

亚型

IgG

不含载体蛋白

反应种属

Mouse

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CXCL13 also known as B cell-attracting chemokine 1 (BCA-1) is a chemokine with a molecular weight of approximately 11 kDa. This protein plays an important mechanical role in the immune system by guiding the movement of B cells via chemotaxis. CXCL13 expression occurs mainly in secondary lymphoid tissues such as the spleen lymph nodes and mucosa-associated lymphoid tissue. Its expression is also noted in certain non-lymphoid tissues under inflammatory conditions.

Biological function summary

In the context of immune response and lymphoid tissue organization CXCL13 attracts B cells by interacting with the chemokine receptor CXCR5. These interactions support the formation of B-cell follicles essential for efficient immune responses. CXCL13 is not known to be part of a larger protein complex but instead works through its specific receptor-ligand interactions. Its role in guiding cell migration is vital for the development of secondary lymphoid structures.

Pathways

CXCL13 is significantly involved in the B-cell receptor signaling pathway. The chemokine facilitates the homing of B cells to follicles an important step in the adaptive immune response. CXCL13 closely associates with CXCR5 its specific receptor playing a part in establishing the spatial organization of lymphoid tissues. Additionally the chemokine influences the germinal center reaction an integral part of the immune pathway for antibody maturation.

CXCL13 levels have been linked to autoimmune diseases like rheumatoid arthritis and certain types of lymphomas. In rheumatoid arthritis increased CXCL13 expression correlates with disease severity related to its role in recruiting B cells that contribute to chronic inflammation. In lymphomas particularly those involving B cells CXCL13 supports the growth and survival of malignant cells. Its association with disease states often involves changes in CXCR5 expression or function contributing to disrupted B-cell localization and abnormal immune responses.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in directing the migration of B-lymphocytes to follicles in secondary lymphoid organs.

See full target information Cxcl13

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 12:1587391 PubMed40860911

2025

Melatonin ameliorates zearalenone-induced ovarian damage in mice through antioxidative effects.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Song,Zhong Guo,Lei Song,Jianxiu Ma,Zhifang Zhao,Yanqing Ma,Xiaoyue Ma,Wenjie Jiang,Wanjing Wang,Chongran Liu,Tongtong Wei,Ling Fu,Zhengli Qi,Jin Zhao

Nature communications 16:2152 PubMed40038288

2025

Single cell immunoprofile of synovial fluid in rheumatoid arthritis with TNF/JAK inhibitor treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyang Xia,Chenjia He,Zhinan Xue,Yuelan Wang,Yun Qin,Zhixiang Ren,Yupeng Huang,Han Luo,Hai-Ning Chen,Wei-Han Zhang,Li-Bin Huang,Yunying Shi,Yangjuan Bai,Bei Cai,Lanlan Wang,Feng Zhang,Maoxiang Qian,Wei Zhang,Yang Shu,Geng Yin,Heng Xu,Qibing Xie

Cell proliferation 58:e13740 PubMed39219056

2024

Oncolytic herpes simplex virus propagates tertiary lymphoid structure formation via CXCL10/CXCR3 to boost antitumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Jie Zhang,Wen-Ping Lin,Qing Wang,Shuo Wang,An Song,Yuan-Yuan Wang,Hao Li,Zhi-Jun Sun

JCI insight 9: PubMed38478516

2024

A distinct tumor microenvironment makes anaplastic thyroid cancer more lethal but immunotherapy sensitive than papillary thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Zhen Han,Wei-Dong Ye,Peng-Cheng Yu,Li-Cheng Tan,Xiao Shi,Xu-Feng Chen,Cong He,Jia-Qian Hu,Wen-Jun Wei,Zhong-Wu Lu,Ning Qu,Yu Wang,Qing-Hai Ji,Dong-Mei Ji,Yu-Long Wang

iScience 25:105511 PubMed36437877

2022

Across-cancer specific immune responses induced by nanovaccines or microvaccines to prevent different cancers and cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Diao,Lin Ma,Junping Cheng,Yunzhi Pan,Zuofu Peng,Lianjun Zhang,Minghao Xu,Yupei Li,Xuenong Zhang,Honglv Jiang,Guoqiang Xu,Fenghua Meng,Zhiyuan Zhong,Mi Liu

Immunity 55:115-128.e9 PubMed35021053

2022

TGF-β-mediated silencing of genomic organizer SATB1 promotes Tfh cell differentiation and formation of intra-tumoral tertiary lymphoid structures.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo A Chaurio,Carmen M Anadon,Tara Lee Costich,Kyle K Payne,Subir Biswas,Carly M Harro,Carlos Moran,Antonio C Ortiz,Carla Cortina,Kristen E Rigolizzo,Kimberly B Sprenger,Jessica A Mine,Patrick Innamarato,Gunjan Mandal,John J Powers,Alexandra Martin,Zhitao Wang,Sumit Mehta,Bradford A Perez,Roger Li,John Robinson,Jodi L Kroeger,Tyler J Curiel,Xiaoqing Yu,Paulo C Rodriguez,Jose R Conejo-Garcia

View all publications

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