Anti-CXCL11抗体(ab9955)
Key features and details
- Rabbit polyclonal to CXCL11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CXCL11抗体
参阅全部 CXCL11 一抗 -
描述
兔多克隆抗体to CXCL11 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human CXCL11 aa 1-100.
Database link: O14625 -
常规说明
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
No preservative, sterile filtered -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 10 kDa).
To detect CXCL11 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 10 kDa). To detect CXCL11 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
靶标
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功能
Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. -
组织特异性
High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 6373 Human
- Omim: 604852 Human
- SwissProt: O14625 Human
- Unigene: 632592 Human
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别名
- b R1 antibody
- b-R1 antibody
- Beta-R1 antibody
see all
图片
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Western blot - Anti-CXCL11 antibody (ab9955)Anti-CXCL11 antibody (ab9955) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 10 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa, 45 kDa, 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
数据表及文件
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Datasheet download
文献 (17)
ab9955 被引用在 17 文献中.
- Kong X et al. A comprehensive profile of chemokines in the peripheral blood and vascular tissue of patients with Takayasu arteritis. Arthritis Res Ther 24:49 (2022). PubMed: 35172901
- Li P et al. Glutathione peroxidase 4-regulated neutrophil ferroptosis induces systemic autoimmunity. Nat Immunol 22:1107-1117 (2021). PubMed: 34385713
- Liang J et al. The role of anlotinib-mediated EGFR blockade in a positive feedback loop of CXCL11-EGF-EGFR signalling in anaplastic thyroid cancer angiogenesis. Br J Cancer 125:390-401 (2021). PubMed: 34088989
- Choi YW et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer. Adv Sci (Weinh) 8:2002497 (2021). PubMed: 33643790
- Zhang J & Hu D miR-1298-5p Influences the Malignancy Phenotypes of Breast Cancer Cells by Inhibiting CXCL11. Cancer Manag Res 13:133-145 (2021). PubMed: 33469358
