重组Anti-CUTC抗体[EPR7929] (ab133762)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7929] to CUTC
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CUTC抗体[EPR7929]
参阅全部 CUTC 一抗 -
描述
兔单克隆抗体[EPR7929] to CUTC -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SH-SY5Y, Jurkat, HeLa and A431 cell lysates. Mouse and rat brain, heart, kidney, and spleen tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
解离常数(KD)
KD = 2.00 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR7929 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133762于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
靶标
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功能
May play a role in copper homeostasis. Can bind one Cu(1+) per subunit. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the CutC family. -
细胞定位
Cytoplasm. Nucleus. The overexpressed protein is detected in the cytoplasm, and depending on the cell line, also in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 51076 Human
- Entrez Gene: 66388 Mouse
- Entrez Gene: 361760 Rat
- Omim: 610101 Human
- SwissProt: Q9NTM9 Human
- SwissProt: Q9D8X1 Mouse
- Unigene: 16606 Human
- Unigene: 20257 Mouse
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别名
- CGI 32 antibody
- CGI32 antibody
- Copper homeostasis protein cutC homolog antibody
see all
图片
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All lanes : Anti-CUTC antibody [EPR7929] (ab133762) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CUTC knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaLanes 1-5: Merged signal (red and green). Green - ab133762 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133762 Anti-CUTC antibody [EPR7929] was shown to specifically react with CUTC in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265860 (knockout cell lysate ab258388) was used. Wild-type and CUTC knockout samples were subjected to SDS-PAGE. ab133762 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-CUTC antibody [EPR7929] (ab133762) at 1/2000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST
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All lanes : Anti-CUTC antibody [EPR7929] (ab133762) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab133762 尚未被引用在任何文献中。