Anti-Cullin 4B/CUL-4B抗体(ab67035)
Key features and details
- Mouse polyclonal to Cullin 4B/CUL-4B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cullin 4B/CUL-4B抗体
参阅全部 Cullin 4B/CUL-4B 一抗 -
描述
小鼠多克隆抗体to Cullin 4B/CUL-4B -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human Cullin 4B/CUL-4B. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_003579, 1 a.a. ~ 913 a.a)
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阳性对照
- WB: Transfected 293T cell lysate ICC/IF: HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab67035于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | |
| ICC/IF | (1) |
| 说明 |
|---|
应用说明
ICC/IF: Use at a concentration of 10 µg/ml.
Note: ab67035 requires Protein A affinity purification prior to ICC/IF application.
WB: 1/500 - 1/1000. Detects a band of approximately 99 kDa (predicted molecular weight: 103 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Note: ab67035 requires Protein A affinity purification prior to ICC/IF application.
WB: 1/500 - 1/1000. Detects a band of approximately 99 kDa (predicted molecular weight: 103 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Defects in CUL4B are the cause of mental retardation syndromic X-linked Cabezas type (MRXC) [MIM:300354]; also known as mental retardation syndromic X-linked type 15. A syndromic form of X-linked mental retardation characterized by severe intellectual deficit associated with short stature, craniofacial dysmorphism, small testes, muscle wasting in lower legs, kyphosis, joint hyperextensibility, pes cavus, small feet, and abnormalities of the toes. Additional neurologic manifestations include speech delay and impairment, tremor, seizures, gait ataxia, hyperactivity and decreased attention span. -
序列相似性
Belongs to the cullin family. -
翻译后修饰
Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8450 Human
- Omim: 300304 Human
- SwissProt: Q13620 Human
- Unigene: 102914 Human
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别名
- CUL 4B antibody
- CUL-4B antibody
- CUL4B antibody
see all
图片
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Western blot - Anti-Cullin 4B/CUL-4B antibody (ab67035)All lanes : Anti-Cullin 4B/CUL-4B antibody (ab67035) at 1/500 dilution
Lane 1 : Transfected 293T cell lysate
Lane 2 : non Transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 103 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Cullin 4B/CUL-4B antibody (ab67035)ab67035, at a concentration 10 µg/ml, staining Cullin 4B/CUL-4B in HeLa cell by Immunofluorescence.
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Western blot - Anti-Cullin 4B/CUL-4B antibody (ab67035)This image is courtesy of an Abreview submitted by Svetlana KhoronenkovaAll lanes : Anti-Cullin 4B/CUL-4B antibody (ab67035) at 1/500 dilution (Blocker 1:1 in PBS + 0.1% Tween for 14 hours at 4°C)
Lane 1 : HeLa whole cell lysate
Lane 2 : Cul4 siRNA in HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : An Alexa Fluor®680-conjugated Goat anti-mouse polyclonal at 1/10000 dilution
Predicted band size: 103 kDa
Blocking Step:LI-COR® Odyssey®Blocking Buffer (50%) for 45 minutes at room temperature.
实验方案
数据表及文件
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Datasheet download
文献 (13)
ab67035 被引用在 13 文献中.
- Dubiel D et al. Latent CSN-CRL complexes are crucial for curcumin-induced apoptosis and recruited during adipogenesis to lipid droplets via small GTPase RAB18. iScience 26:106468 (2023). PubMed: 37091236
- Zeng J et al. A Hypoxia-Induced SCFFBXL1 E3 Ligase Ubiquitinates and Degrades the MEN1 Tumor Suppressor to Promote Colorectal Cancer Tumorigenesis. Cancer Res Treat 54:525-540 (2022). PubMed: 34237211
- Jones TM et al. Targeted CUL4A inhibition synergizes with cisplatin to yield long-term survival in models of head and neck squamous cell carcinoma through a DDB2-mediated mechanism. Cell Death Dis 13:350 (2022). PubMed: 35428778
- Qiu H et al. Retracted: Long non-coding RNA TP73-AS1 facilitates progression and radioresistance in lung cancer cells by the miR-216a-5p/CUL4B axis with exosome involvement. Thorac Cancer 12:409 (2021). PubMed: 32841514
- Weng G et al. Tetramethylpyrazine Improves Cognitive Function of Alzheimer's Disease Mice by Regulating SSTR4 Ubiquitination. Drug Des Devel Ther 15:2385-2399 (2021). PubMed: 34103899
